Effect of Different Concentrations of Iron Oxide Nanoparticles on Expression of p53 Gen in Human Derived amniotic Membrane Mesenchymal Stem Cell

Background: Paramagnetic nanoparticles (SPIONs) have made extensive advances in nanotechnology. The unique prop-erties of these particles have expanded their application in vari-ous fields, including medical fields. One of these uses is non-invasive analysis for cell tracking. However, the possibility of toxicity in cells is reported by these nanoparticles. Due to the fact that cellular damage caused by iron oxide nanoparticles is concentration-dependent. So finding the appropriate concentra-tion of iron oxide nanoparticles is very important to prevent cell damage or cell death caused by apoptosis. The aim of this study was to find a concentration of SPIONs that does not result in apoptosis in cells. Therefore, this study aimed to investigate the effects of different concentrations of iron oxide nanoparticles on cell survival, the effect on increased gene expression and proteins involved in apoptosis (P53) In human amniotic mem-brane derived mesenchymal stem cells (hAMSCs) were evaluated.Materials and Methods: First, stem cells were extracted from human amniotic membrane tissue and cultured. Then, the vi-ability of the cells treated with different concentrations of iron oxide nanoparticles (200, 150, 100, 50, 0 μg / ml) over a period of 24 hours was evaluated by MTT method. The effect of con-centrations of 0, 100,150 and 200 μg / ml of nanoparticles after 24 hours in hAMSCs was investigated for expression of P53 gene by Real-Time PCR. Further, the expression of P53 protein expression in cells treated with nanoparticles Iron oxide was measured using western blot method.Results: Iron oxide nanoparticles had no significant effect on cell survival at concentrations of 50 and 100 μg / ml in 24 hours, while cell survival was significantly reduced at concentrations of 150 and 200 μg / ml. The results of qRT-PCR analysis showed that the expression of P53 gene increased at a concentration of 150 μg / ml and above, significantly. In addition, Western Blot results showed that the expression of P53 protein also increased in cells treated with 150 and 200 μg / ml concentrations of na-noparticles.Conclusion: According to the results, the nanoparticles used in this study are appropriate at concentrations ≤ 100 μg / ml for cell tracking.

Keywords:

Amniotic Membrane Stem Cells , Iron Oxide Nano-particles , Apoptosis , P53 Gene , Spions