Association between Altered Titers of Antiphospho-lipid Antibodies and Recurrent Implantation Failure

Background: Recurrent implantation failure (RIF) is failure to achieve clinical pregnancy after transfer of three good-quality embryos in a minimum of three fresh/frozen cycles in a woman under the age of 40 years. Immunological causes of RIF are of controversial aspects of implantation, mainly because antiphos-pholipid antibodies (aPL) are an important cause of recurrent pregnancy loss. So, the emerging role of aPL on implantation and pregnancy complications led us to investigate these anti-bodies in RIF patients.Materials and Methods: For this respect a pilot study was performed on 30 RIF women referred to Royan Institute and 10 healthy women with at least one child (control group). RIF patients had no immunological desease. Consent was obtained from all women according local ethical approval then, blood samples were collected from them (blood collection of RIF pa-tients was through ART procedure). Quantitative detection of IgG/IgM antibodies against cardiolipin, B2GPI, phosphatidyl ethanolamin and phosphatidyl-serine plus native human pro-thrombin was conducted using ELISA immunoassay. Also lu-pus anti-coagulant test was performed on all samples.Results: The results showed that titters of antibodies against cardiolipin, B2GPI and phosphatidyl ethanolamin were higher in RIF patients vs. controls. Titer of anti phosphatidylserine-prothrombin antibody in RIF women was significantly higher than healthy women. Results of lupus anti-coagulant test were negative for all participants.Conclusion: These findings showed association between al-tered levels of antiphospholipid antibodies titers with recurrent implantation failure and women infertility.

Keywords:

RIF , Antiphospholipid Antibodies , ELISA