Investigating The Expression of Mir34c in Testis Tis-sue of Cholestatic Male Rats

Publish Year: 1398
نوع سند: مقاله کنفرانسی
زبان: English
View: 375

نسخه کامل این Paper ارائه نشده است و در دسترس نمی باشد

  • Certificate
  • من نویسنده این مقاله هستم

استخراج به نرم افزارهای پژوهشی:

لینک ثابت به این Paper:

شناسه ملی سند علمی:

RROYAN20_379

تاریخ نمایه سازی: 29 مهر 1398

Abstract:

Background: Cholestasis is a pathophysiological condition that is occurred due to various reasons such as gallstone, malig-nancy or genetic factors. Histological studies have been shown that cholestasis reduces proliferation of spermatogenic cells, causes apoptosis, and prevents the maturation of germ cells, in men. microRNAS (miRNAs) are non-coding sequences that have several regulatory roles in cell. Several evidences have been suggested the role of miRNAs during spermatogenesis and their importance in male fertility. miR34c has a specific and high expression in adult male testis. Mir34C and its related genes, are important mediator of self-renewal and differentia-tion of germ cells and have been considered as new targets in fertility problems.Materials and Methods: Eight adult male wistar rats were di-vided into two groups named as control and cholestatic (n=4 each). To induce obstructive cholestasis their common bile duct was closed by surgery and the expression of miR34C in testis tissue of cholestatic and control rats, was assessed by quantitive real time PCR (qRT-PCR) technique. First, the primer sequence for mir34c and U6 was designed and then, the total RNA was extracted from the tissues and converted to cDNA. The syn-thesized cDNA was used to carry out the reverse transcription reaction and the relative expression of mir34C was determined using R=2^(- (∆∆CT)) formula.Results: The results of qRT-PCR showed that the expression of miR34c in the cholestasis group decreased by compared to the control group. This result showed that cholestasis reduced the expression of miR34C in testicular tissue. According to statisti-cal analysis, this difference was significant.Conclusion: Previous studies demonstrated that expression of miR34c is disrupted in pathological conditions of testis such as oligospermia and azoospermia and may be led to interfere with self-renewal and differentiation of germ cells. Our results also indicated the reduced expression of miR34c during cholestasis. Reducing miR34c expression in cholestasis animals may sug-gest that cholestasis has been able to disturb the expression of miR34C in the testis tissue and possibly through this change leads to disruption of the spermatogenesis process.

Authors

AH Hasani Fard

Department of Animal Biology, Faculty of Biological Sciences,Kharazmi University, Tehran, Iran

H Mohseni Kouchesfahani

Department of Animal Biology, Faculty of Biological Sciences,Kharazmi University, Tehran, Iran

H Jalali

Department of Animal Biology, Faculty of Biological Sciences,Kharazmi University, Tehran, Iran