Effect of Curcumin and Nanocurcumin on The Ex-pression Level of Prominent Developmental Gene (TIMP-1) in Endometrial Tissue Culture of Women with Polycystic Ovary Syndrome.

Background: Poly Cystic Ovary Syndrome (PCOS) is a com-mon endocrinological disorder. This syndrome affects 5–7% of women at their reproductive ages. It considered as a complex disorder with interaction of genetic and environmental factors. This syndrome is associated with: insulin resistance, hyperan-drogenemia, chronic inflammation and oxidative stress. Endo-metrium undergoes a complex series of organized changes in each menstrual cycle. It is receptive in a short period of time which is known as window of implantation (WOI). There is increasing evidence of dysregulated expression of markers of uterine receptivity in endometrium of women with PCOS. TIMP-1 has showed crucial role in reproductive organs and its decreasing level has been detected in follicular fluid of women with PCOS.Materials and Methods: In this study, endometrial biopsy of a woman with PCOS has been cultured in vitro and after the sec-ond passage, the cells were exposed to 4 different treatments: curcumin, nanocurcumin (DNC), carrier (Sham), DMSO (Sham), paralleled to untreated group. By MTT assay the best concentration of curcumin and nanocurcumin and carrier were chosen. After 48 hours of treatment each 5 group’s cells were snap frizzed separately and were kept in -70 °C. After cDNA synthesis, the quantification of TIMP-1 expression evaluated by real-time PCR.Results: Our data demonstrated that the treated cells showed significant decrease in TIMP-1 expression for both curcumin (P value = 0.004) and nanocurcumin (P value = 0.05) compared to untreated group.Conclusion: This primary data indicates that curcumin and na-nocurcumin can influence on gene expression profile of endo-metrial cells of a woman with polycystic ovary syndrome. The data needs further investigation.

Keywords:

PCOS , Timp-1 , Implantataion , Curcumin , Nanocur-cumin