Factors Influencing In Vitro Flowering from Styles of Saffron

This paper refers to the factors affecting in vitro flowering as follows in turn: the important factor was explants age according to style length, the second was culture condition containing temperature and light, the third was growth regulator. Style incised from 55-70 mm length floral buds with light blue perianths as explants cultured on media regulated with 26.8 µM 1-naphthaleneacetic acid and 31.1 µM 6-Benzylaminopurine in the dark at 20 ℃ was optimal condition for in vitro flowering induced, the induction ratio was 37.5-40.0 %. As for growth regulator only, the higher 6-BA/NAA in the dark at 20 ℃ was beneficial to in vitro flowering induced.

Keywords:

Crocus sativus , explants age , growth regulator , style , temperature