COI code: SRCCG05_098
Paper Language: English
How to Download This Paper
For Downloading the Fulltext of CIVILICA papers please visit the orginal Persian Section of website.
Authors Down-regulation of anti-apoptotic genes in tumor cell lines is facilitated by suppression of OCT4B1mr Mirzaei - Molecular Medicine Research Center, Rafsanjan University of Medical Sciences, Rafsanjan, Iran
m mahmoodi - Molecular Medicine Research Center, Rafsanjan University of Medical Sciences, Rafsanjan, Iran
g Hassanshahi - Molecular Medicine Research Center, Rafsanjan University of Medical Sciences, Rafsanjan, Iran
z Ahmadi - Molecular Medicine Research Center, Rafsanjan University of Medical Sciences, Rafsanjan, Iran
Abstract:Back ground and Aim: The OCT4B1 as a variant of OCT4 is expressed in both cancer cells and tissues. The anti-apoptotic property of this variant aid cancer cells to escape fromapoptosis. Therefore, the aim of the present study was to determine the effects of OCT4B1 suppression on regulation of 25 genes involved in anti-apoptotic pathway in tumor cell .lines Material and methods: AGS gastric adenocarcinoma , 5637 bladder tumor and U-87MG brain tumor cells were transfected with specific OCT4B1 siRNA and a scramble siRNA by siRNA silencing gene technology, using Lipofectamine 2000 commercial kit. The real-time PCR technique was employed to examine and calculate fold changes of evaluated genes .using the 2−ΔΔCT formula Results: Present results demonstrated that 22 88% of interested genes were similarly down-regulated in all three examined cell lines. Our results also indicated that three genes CASP2, IGF1R,TNF were up-regulated. The CFLAR gene was down-regulated in AGS, while it was inversely up-regulated in 5637 and U87MG cells Conclusions: It may possibly be concluded that suppression of OCT4B1 can lead to apoptosis in tumor cell lines and this is at least facilitated via down-regulation of examined anti-apoptotic genes. Accordingly, suppression of OCT4B1 may probably be considered as useful tool in cancer therapy and research
COI code: SRCCG05_098
how to cite to this paper:If you want to refer to this article in your research, you can easily use the following in the resources and references section:
Mirzaei, mr; m mahmoodi; g Hassanshahi & z Ahmadi, 2019, Down-regulation of anti-apoptotic genes in tumor cell lines is facilitated by suppression of OCT4B1, 5th congress of student research committee of rafsanjan university of medical sciences, رفسنجان, دانشگاه علوم پزشكي رفسنجان, https://www.civilica.com/Paper-SRCCG05-SRCCG05_098.htmlInside the text, wherever referred to or an achievement of this article is mentioned, after mentioning the article, inside the parental, the following specifications are written.
First Time: (Mirzaei, mr; m mahmoodi; g Hassanshahi & z Ahmadi, 2019)
Second and more: (Mirzaei; mahmoodi; Hassanshahi & Ahmadi, 2019)
For a complete overview of how to citation please review the following CIVILICA Guide (Citation)
The University/Research Center Information:
Type: Medical University
Paper No.: 288
in University Ranking and Scientometrics the Iranian universities and research centers are evaluated based on scientific papers.
Research Info Management
Export Citation info of this paper to research management softwares
Iran Scientific Advertisment Netword
Share this paper
WHAT IS COI?
COI is a national code dedicated to all Iranian Conference and Journal Papers. the COI of each paper can be verified online.