Allantion improves apoptosis in methionine-choline deficient (mcd) diet induced nonalcoholic steatohepatitis in mice

Objectives: non-alcoholic fatty liver disease(NAFLD) is one of the most common liver diseases worldwide.it has been estimated the NAFLD involves approximately one third of the population in western countries,20%of whom develop into the NASH . Allantoin is one of the principal compounds in many plants and is a natural, safe ,and non_toxic compound .The wound healing and tissue regeneration effects of allantoin are previously well known .This study was designed to assess the effects of allantoin on MCD diet induced NASH apoptosis in mice. Methods: C57\BL6 male mice received saline and allantoin as the control groups. NASH was induced by methionine-choline deficient diet (MCD).In the NASH+allantoin group, allantoin was injected in the mice feeding on an MCD diet .After etherize ,abdomen was excised and the liver was removed. Real-time RT-PCR were performed. Results: Allantoin administration decreased Bax\Bcl12 ratio,caspase3 and P53 in the NASH group. Conclusion: This study indicated that allantoin could improve animal induced NASH by changes in the expression of apoptosis related genes.

Keywords:

Allantoin , Fatty liver disease , NAFLD , NASH