The evaluation of Ceruloplasmin(Cer), Zinc(Zn) and Total Antioxidant Capacity(TAC) in elderly and healthy smoker drivers as compared to non-smoker drivers

Introduction: Smokers are exposed to significant amounts of oxidant and free radicals. The oxidative stress based on cigarette smoke plays an important role in the pathogenesis of many diseases, including of cancer and cardiovascular disease. In the present study, changes in concentration of Cer, Zn and TAC in the blood samples of elderly and healthy smokers are compared with non-smokers subject . Materials and Methods: In this case-control study, Cer, Zn and TAC levels were evaluated in smoker drivers (30 healthy smoker drivers with 45-70 age and 30 non-smoker drivers) . For evaluation of Ceruloplasmin is used from Immunotorbideometric method and using BIONIK kit and Hitachi 104 auto analyzer. Zinc measurements were performed using a zinc kit by photometric method. To evaluate of TAC, the ELISA method was used with zellbio kit. Data were analyzed by means comparison and standard deviation using of SPSS. The mean of data was assessed in two groups with independent T-Test. The significance levels was considered with p <0.05. Result: Comparison of the parameters in smokers and non-smokers showed that the changes in TAC, Cer and Zn levels were significant at the level of p<0.01. In addition, the research results showed that the TAC levels in smokers were 0.444±0.085 which compared to non-smokers to the 0.885±0.641 decreased. Conclusion: The results of our research showed that oxidative stress caused by smoke with TAC and Zn decreased and Ceruloplasmin increased. These changes, in the long run, it can lead to the development of cardiovascular diseases.

Keywords:

Cigarette , Oxidative stress , Total Antioxidant Capacity , Zinc , Ceruloplasmin