CIVILICA We Respect the Science
Publisher of Iranian Journals and Conference Proceedings
Paper
title

Experimental evaluation of the hemodynamic changes induced with intravascular injection of the Echis carinatus venom in rats

Credit to Download: 0 | Page Numbers 1 | Abstract Views: 63
Year: 2019
COI code: TOXICOLOGY15_021
Paper Language: English

How to Download This Paper

For Downloading the Fulltext of CIVILICA papers please visit the orginal Persian Section of website.

Authors Experimental evaluation of the hemodynamic changes induced with intravascular injection of the Echis carinatus venom in rats

  Hossein Fatemkiai - Department of Physiology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
  Ramin Seyedian - Department of Pharmacology, Bushehr University of Medical Sciences, Bushehr, Iran

Abstract:

Introduction: Echis carinatus (E.carinatus) is recognized, as one of the most dangerous snakes globally that inhabits in Iran. In this study, the hemodynamic responses of this venom were evaluated in rats. The neutralizing effects of the Iranian polyvalent antivenom and different drugs were assessed. In addition, the induced pathological malformations in different organs were investigated. Methods: Animals were anesthetized and placed supine on the surgical table. Administration of the venom and drugs (heparin, epinephrine, atropine, ketorolac, dexamethasone, polyvalent antivenom) were performed via a cannula that was placed in to the left femoral vein. The arterial cannula was connected to a pressure transducer (MLT844, AD instruments, Australia) for continuous recording of the hemodynamic changes by means of a powerlab acquisition system. At the end of the study, the animals were killed with cervical dislocation and their internal organs (heart, kidney, liver, and the lungs) preserved for pathological analysis in formalin solution (10%).Results: Injection of the E.carinatus venom (40 μg per rat) intravenously caused a prominent hypotension subsequent to transient hypertension leading to the death in few minutes accompanied with the marked decrease in the heart rate. This venom had no arrhythmogenic activity while there was decrease of the pulmonary rate within the experiment. Pretreatment with the Iranian polyvalent antivenom (pepsin derived product), 10 minutes before venom injection, neutralized the hemodynamic responses while this remedy had no effect when instilled two minutes after venom injection. Heparin (300IU/kg) and epinephrine (0.5μg per rat) prevented hemodynamic responses when used 10 minutes before venom instillation while atropine (1 mg/kg), dexamethasone (1 mg/kg) and ketorolac (10mg/ml) had no effects. Histologically the lung was the most vulnerable organ with mononuclear infiltration, microcystic formation and significant capillary congestion. There was prominent renal pathological deteriorations including mesangial cell infiltration and diffuse bleeding leading to the acute tubular necrosis.Conclusion: This experimental study demonstrate that injection of the E.carinatus venom intravenously in one minute, causes hypotension leading to death, bradycardia and decrease in pulmonary rate without histologically significant changes in the heart. In future studies, the cardiotropic potencies of this venom on the rat isolated heart muscle should be investigated.

Keywords:

Perma Link

https://www.civilica.com/Paper-TOXICOLOGY15-TOXICOLOGY15_021.html
COI code: TOXICOLOGY15_021

how to cite to this paper:

If you want to refer to this article in your research, you can easily use the following in the resources and references section:
Fatemkiai, Hossein & Ramin Seyedian, 2019, Experimental evaluation of the hemodynamic changes induced with intravascular injection of the Echis carinatus venom in rats, 15th iranian congress of toxicology, تهران, دانشگاه علوم پزشكي شهيد بهشتي, https://www.civilica.com/Paper-TOXICOLOGY15-TOXICOLOGY15_021.htmlInside the text, wherever referred to or an achievement of this article is mentioned, after mentioning the article, inside the parental, the following specifications are written.
First Time: (Fatemkiai, Hossein & Ramin Seyedian, 2019)
Second and more: (Fatemkiai & Seyedian, 2019)
For a complete overview of how to citation please review the following CIVILICA Guide (Citation)

Scientometrics

The University/Research Center Information:
Type: Medical University
Paper No.: 2097
in University Ranking and Scientometrics the Iranian universities and research centers are evaluated based on scientific papers.

Research Info Management

Export Citation info of this paper to research management softwares

Iran Scientific Advertisment Netword

Share this paper

WHAT IS COI?

COI is a national code dedicated to all Iranian Conference and Journal Papers. the COI of each paper can be verified online.