Determination of natamycin and sorbate preservatives in doogh samples by developing a high performance liquid chromatography method

Publish Year: 1398
نوع سند: مقاله کنفرانسی
زبان: English
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TOXICOLOGY15_103

تاریخ نمایه سازی: 15 بهمن 1398

Abstract:

Introduction: Natamycin and potassium sorbate are commonly used preservatives with inhibitory effects on various types of microorganisms such as bacteria, yeasts, and molds. However, they are forbidden to use in doogh (traditional Iranian yoghurt drink) production in Iran. In this research, a fast and simple method was developed for simultaneous determination of natamycin and potassium sorbate in doogh samples by reverse phase liquid chromatography (RP-HPLC).Methods: A chromatographic system from Agilent technology coupled to diode array detector (DAD) at 255 nm and 307 nm was applied for detection of sorbate and natamycin, respectively. The stationary phase was ZORBAX Eclipse-XDB C18 and the mobile phase (at a flow rate of 1.0 mL/min) consisted of an ammonium acetate buffer and acetonitrile (78:28 v/v).Results: The calibration curves of the studied chemicals were linear in the range of 0.5-50.0 μg/mL with a determination coefficient (R2) 0.9999 for both compounds. The average recoveries at 3 spiked levels (2.0, 10 and 40 μg/mL) were in the range of 102-118% and 106-118% for sorbate and natamycin respectively, with a satisfactory precision (RSD<20%). The limit of detection (LOD) and limit of quantification (LOQ) were 0.031 and 0.102 μg/mL for natamycin and 0.025 and 0.082 μg/mL for sorbate, respectively.Conclusion: The method was successfully applied for determination of natamycin and sorbate in 12 doogh samples collected from Tehran market. The results showed that none of the samples were contaminated with natamycin or sorbate preservatives.

Authors

M Mottaghi

Department of Toxicology and Pharmacology, School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran

F Peiravian

Department of Pharmacoeconomic and Pharmaceutical Administration, School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran

Z Mardani

Department of Pharmacoeconomic and Pharmaceutical Administration, School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran

A Shakoori

Vice-Chancellor for Food and Drug Affairs, Shahid Beheshti University of Medical Sciences, Tehran, Iran