Evaluation of Klebsiella Pneumoniae Carbapenemase-Producing Isolated from Wound Infectious in Isfahan, Iran
Publish Year: 1398
نوع سند: مقاله کنفرانسی
زبان: English
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شناسه ملی سند علمی:
WTRMED06_021
تاریخ نمایه سازی: 5 بهمن 1398
Abstract:
Background: Klebsiella pneumoniae is one of the most important opportunistic enteric bacteria and is a major cause of pneumonia and wound infectious. Potential of Klebsiella pneumoniae to produce K. pneumoniae Carbapenemase (KPC) is an important to hydrolyze carbapenems and other beta-lactam antibiotics. Carbanemase-producing bacteria can be characterized by Modified Hodge Test (MHT) and use of boronic acid as a KPC inhibitor. So we decided to identify KPC in K. pneumoniae isolated from bedsore wound infectious hospitalized in ICU of Isfahan.Methods: In this cross sectional study, 60 K. pneumoniae strains were isolated from bed sore wound infections of patients hospitalized in Isfahan. Biochemical tests were selected for K. pneumoniae characterization. Carbapenem-resistant was analyzed by disc diffusion method according to CLSI 2011 guideline. MHT and Boronic acid method was used for detection of Klebsiella pneumoniae carbanemase-producing which were examined by PCR method.Results: 53% of 60 isolates were resistant to carbapenem according to CLSI breakpoints and 27% were susceptible (P<0.05). MHT was positive in all of carbapenem-resistant isolates. According to results there were no synergism effect between boronic acid and meropenem and MHT has not enough specificity for detection of KPC and also 34% of isolates carried blaKPC gene.Conclusion: According to the results of this study, MHT is an important screening for detection of KPC in producing K. pneumoniae specifically in the patient with bed sore wound infections.
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Authors
Fahimeh Nourbakhsh
Ph.D, Student of Medical Bacteriology, Medical Toxicology Department of Pharmacodynamics and Toxicology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran