Combination of Wharton‘s jelly derived mesenchymal stem cells conditioned medium and collagen Scaffold can introduce a new therapeutic potential for wound healing

Recently various stem cells derived conditioned medium are at great interest because of their different therapeutic potentials and greater safety in comparison with stem cells. Combination of these conditioned media with various kind of scaffolds can also mimic the environment of cell in human body. Wharton jelly mesenchymal stem cells derived conditioned medium contains growth factors such as VEGF, IGF and FGF which are the potent factors for tissue damage and wound healing. Collagen scaffolds due to their known advantages for skin disorders can be the best candidates for this combination. In the current study we evaluated the effect of combination of the Wharton‘s jelly mesenchymal stem cell derived conditioned medium and collagen scaffold on physical and biological properties of collagen scaffolds for tissue engineering application. Wharton‘s jelly mesenchymal cells were cultured using DMEM, supplemented with 10% (v/v) FBS, 10 μg/mL streptomycin and 100 mg/mL penicillin then conditioned medium were obtained using FBS free media for 72 hours and added to prepared collagen scaffolds according to the protocol. Collagen scaffold was evaluated by Fourier transforms infrared spectrometry (FTIR) and scanning electron microscopy (SEM) analysis. The viability and proliferation rate of keratinocyte on scaffold was also evaluated. The investigation showed that the scaffold was highly porous with interconnection. The adhesion, viability and proliferation of keratinocyte was remarkable. This combination can be a suitable choice for therapeutic purposes, mainly wound healing according to physical and biological properties of the collagen scaffold.

Keywords:

collagen scaffold , conditioned medium , Wharton‘s jelly mesenchymal stem cells