Antibacterial nanofibrous mats from PCL/Gelatin/Clindamycin for wound dressing applications

Nowadays, nanofibers are used in different fields for various applications one such application is in drug delivery, wound dressing, because of their good characteristics such as high permeability, and specific surface area. For better performance natural and synthetic polymers like fibroin, gelatin, polycaprolactone (PCL) and polyacrylamide (PAA) are electrospun to form a blend. Antibiotics such as linezolid, enrofloxacin and vancomycin are used in the solution to impart antibacterial properties to the electrospun mats applied for wound dressing. In this study, nanofibers were produced from PCL (15%) and gelatin (Gel) (15%) with 25:75 ratios. Acetic acid was used as co-solvent. Clindamycin with different percentages (2, 4 and 6% W/V) was added to Gel and PCL polymeric solution. Surface morphology and functional groups of nanofibers were examined using SEM and FT-IR spectroscopy, respectively. The antibacterial properties of nanofibers were evaluated quantitatively. SEM images of scaffolds showed uniform and bead-free morphology that with incorporating the drug, the diameters of nanofibers reduced. The FT-IR confirmed the presence of drug in the mats. Additionally, the fibers demonstrate proper antibacterial properties against a gram-positive and a gram-negative bacterium. The cell culture data indicated good proliferation of the cells on the web. The release of the drug was investigated by degradation studies

Keywords:

Electrospinning , Gelatin , Polycaprolactone , Clindamycin , Wound dressing