Evaluation of PGA/Collagen and PGA/Collagen/Bioglass conduits in peripheral nerve defect regeneration

Publish Year: 1398
نوع سند: مقاله کنفرانسی
زبان: English
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WTRMED06_174

تاریخ نمایه سازی: 5 بهمن 1398

Abstract:

Providing a solution for the treatment of damaged tissues in the body is one of the challenges that medical science has been facing from late to now. Peripheral nerve injury can considerably affect the daily life of injured people through reduced function and permanent deformation of the nerve. Therefore, the main purpose of neural tissue and medical engineering is developing of biomedical alternatives to restore, maintain or improve tissue damage and organ function.In this study, a novel conduit based on polymer blend nanocomposites of polyglycolic acid (PGA), collagen, and nanobioglass (NBG) were prepared by electrospinning technique and then compared with PGA/collagen and PGA conduits. Additionally, their various properties were characterized by scanning electron microscopy (SEM), X‐ray diffraction (XRD), contact angle, dynamic mechanical thermal analysis (DMTA), tensile strength, Fourier‐transform infrared (FTIR), porosity, degradation, MTT assay and DAPI staining. For evaluating the ability of the conduits in sciatic nerve regeneration, following confirmation of suitability of the fabricated conduits, they were sutured in a 10mm Rat sciatic nerve gap. Finally, the nerve regeneration process was evaluated by functional tests (SFI, EMG and Hot plate test), histological tests of the reconstructed nerve (including toluidine blue staining, H & E) and immunohistochemistry of (NF-200, S100 and CD31 antibodies). SEM analysis showed appropriate range of the nanofibers diameter and the pore size of the electrospun mat. According to the results of porosity test, suitable porosity was observed in the conduits. Based on the XRD analysis, amorphous structure of the bioglass nanoparticle showed. FTIR analysis indicated good interaction between polymer–polymer macromolecules and polymer particles. The contact angle test showed that PGA/Collagen/NBG conduits were more hydrophilic than PGA/Collagen and pure PGA conduits. The results of the mechanical test showed that PGA/Collagen/NBG conduits have better elasticity, good performance and ideal resistance to collapse and tensile strength compared to pure PGA and PGA/Collagen conduits. According to MTT assay and DAPI staining technique, nanofibrous electrospun PGA/collagen/NBG conduits are more suitable for cell adhesion and proliferation in comparison with either PGA or PGA/collagen conduits. The results of the functional tests (EMG, Hot plate test, SFI) and histological tests of the reconstructed nerve (including Toluidine blue and H& E staining) and immunohistochemistry of (NF-200, S100 and CD31) indicated significant improvement in rats receiving conduits specially in PGA/Collagen/NBG group.The results of different In-vitro and In-vivo tests showed that the PGA/Collagen/NBG conduits were more favorable than PGA/Collagen and PGA in sciatic nerve regeneration and these conduits have potential for nerve regeneration.

Authors

Navid Dehnavi

PHD, prof. of developmental biology, science and research branch of Islamic azad university, Tehran, Iran