Ali Reza Taheri - Islamic Azad University – Ilam Branch, Ilam, Iran
Nasrin Rahmatian - Islamic Azad University – Ilam Branch, Ilam, Iran

The selective molecularly imprinted polymer was produced using Azathioprine and methacrylic acid as a template molecule and monomer, respectively. In order to investigate the effect of independent variables on the extraction efficiency, the response surface methodology (RSM) based on Box-Behnken design (BBD) was employed. The analytical parameters such as precision, accuracy and linear working range were also determined in optimal experimental conditions and the proposed method was applied to analysis of Azathioprine. The linear dynamic range and limits of detection were 2.5-0.01 and 0.008 mg L-1 respectively. The recoveries for analyte were higher than 95% and relative standard deviation values were found to be in the range of 0.83-4.15%. This method was successfully applied for the determination of Azathioprine in biological and pharmaceutical samples

Azathioprine, Nano molecularly imprinted polymer, Solid-phase extraction, Spectrophotometry, Box–Behnken design