Fatemeh Bagheri - Department of Plant Breeding, Ahvaz Branch, Islamic Azad University, Ahvaz, Iran
Mahdi Soltani Howyzeh - Department of Plant Breeding, Ahvaz Branch, Islamic Azad University, Ahvaz, Iran
Vahid Shariati - National Institute of Genetic Engineering and Biotechnology, Tehran, Iran

In the cucurbitaceae family, transcriptome profiling data of the flower development are limited to the determination of the flower s sex, which in the different developmental phases, several genes are expressed differently or specifically. In this family the most species are unisexual florals. Among 800 studied species, 460 are monoecious and 340 are dioecious. Citrullus colocynthis L. is a perennial plant, with male and female flowers on a monoecious. In monoecious, CsACS2 gene increase ethylene production which leads to female florals and the andromonoecious, in other hands, CmACS-7 gene controls stamina development in female florals. The bioinformatics analysis was carried out using the Sequence Viewer CLC-7 software for the six species plant of the Cucurbitaceae family to study the CsACS2 gene sequences from the obtained data. The alignment of CsACS2 gene showed high similarity in the domains. Also, pattern of the phylogeny tree showed close phylogeny relations between the studied species.

Citrullus colocynthis L., Inflorescence sexuality, CsACS2, Bioinformatics analysis.