Nasrin Noshadi - Marvdasht Azad Universtiy
Asghar Arshi - Young Researchers and Elite Club, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran

Background: microRNAs are short non-coding RNAs (20-24 nt) that are involved in post-transcriptional regulation of gene expression in multicellular organisms by affecting both the stability and translation of mRNAs. miR-30-3p has been reported to be a tumor suppressor and a promising therapeutic target in cancer. miR-30-3p has been associated with epithelial mesenchymal transition and chemo-resistance in cancer. The aim of this study is to investigate the expression of miR-30-3p, its prognostic roles and its potential targets in breast cancer by RT-qPCR.Methods: In the present case-control study, 20 tissue samples from patients with breast cancer and 20 tissue samples from healthy group under the direct supervision of a pathologist specialist due to clinical presentation and laboratory findings were collected. After extracting RNA from normal and tumor blood samples, cDNA synthesis method according to the protocol and RT-qPCR was performed. mir-30-3p expression level was calculated using Livak method. All data were analyzed using SPSS software.Results: The results of RT-qPCR indicated miR-200b was downregulated in breast cancer tissues and its low-expression correlated with poor outcome in breast cancer patients. Expression level of mir-30-3p was 31.24% and 68.76% in breast cancer patients and healthy group respectively (p value=0.001).Conclusion: Due to the previous reports, mir-30-3p act as tumor suppressor in breast cancer, which could be used as biomarker for breast cancer diagnosis. In this study we propose that miR-30-3p is a potential diagnostic marker and therapeutic target of breast cancer.

breast cancer, microRNA, mir-30-3p, RT-qPCR