Pegah Mohtaj - Department of Industrial and Environmental Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), ۱۴۹۶۵/۱۶۱, Tehran, Iran
Fatemeh Tabandeh - Department of Industrial and Environmental Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), ۱۴۹۶۵/۱۶۱, Tehran, Iran
Mahvash Khodabandeh - Department of Industrial and Environmental Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), ۱۴۹۶۵/۱۶۱, Tehran, Iran
Sarah Khalaj - Department of Industrial and Environmental Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), ۱۴۹۶۵/۱۶۱, Tehran, Iran

Hyaluronic acid (HA) is a natural polysaccharide consist of repeating units of UDP-N-acetyl-glucosamine (UDP-GlcNAc) and UDP-glucuronic acid (UDP-GluA). Although HA is commercially produced by Streptococcus zooepidemicus, its production by GRAS microorganisms is highly recommended. In this study, two recombinant strains of Corynebacterium glutamicum harboring S. zooepidemicus and Pseudomonas putida genes were employed. HA molecular weight (MW) is an important factor which determines its application at different industries. Correlation between hyaluronic acid MW and growth temperature showed that temperature had significant impact on HA MW, but it is remarkably affected by genetic construction of the strain.

hyaluronic acid, hasA, bacterial fermentation, GRAS, molecular weight