The Protective Effect of Celecoxib on CA1 Hippocampal Neurons and Oxidative Stress in a Rat Model of Parkinson’s disease Effect of Celecoxib on CA1 Hippocampal Neurons and Oxidative Stress

Several studies point to an important role of neuroinflammation in Parkinson s disease (PD). Cognitive and memory impairments have been known in the early stages of PD. Objectives: In the present study we examined the effects of celecoxib (CLX), a selective inhibitor of cyclooxygenase-2 (COX-2), on hippocampus cell loss, passive avoidance memory and antioxidant status in a rat model of PD. We used the subcutaneous injection of 2.5 mg/kg/48h rotenone (ROT) for 4 weeks for induction of PD in a male Wistar rat. Animals were randomized to 4 groups (n=12): Control, sham, PD and PD+CLX group that receive celecoxib (20 mg/kg/day) for 4 weeks. Passive avoidance memory evaluated. We also determined protective effect of CLX on number of CA1 neurons in Nissl and TUNEL staining. Total antioxidant capacity (TAC) and malondialdehyde (MDA) a marker of lipid peroxidation in hippocampus assessed. Our findings indicated administration of CLX increase the passive avoidance memory (P < 0.05), and by decrease in apoptosis caused increase in viable pyramidal neurons in CA1 hippocampus (P < 0.01). On the other hand CLX markedly reduced MDA level and increased TAC in hippocampus of PD model animal (p < 0.05). It seems CLX with anti-inflammatory and antiapoptotic effect could prevented from neurons loss and memory impairment which induced in PD.

Keywords:

Celecoxib , Parkinson’s disease , Hippocampus , memory