Masoume Haghbin Nazarpak - New Technologies Research Center (NTRC), Amirkabir University of Technology, P. O. Box:۵۹۱۶۳۳۳۱۱
Farzaneh Pourasgari - New Technologies Research Center (NTRC), Amirkabir University of Technology, P. O. Box:۵۹۱۶۳۳۳۱۱
Mohammahdnabi Sarbolouki - New Technologies Research Center (NTRC), Amirkabir University of Technology, P. O. Box:۵۹۱۶۳۳۳۱۱

Nowadays, there is a significant need for bone replacement materials used in bone tissue engineering. The scaffolds for bone tissue engineering should consider the functional requirements in which composites play a vital role. In this study, porous Poly(lactic-co-glycolic acid)/Hydroxyapatite composites were prepared by freeze-extraction method and coated with triblock copolymer. Human mesenchymal stem cells were cultured on scaffolds and cells adhesion to the scaffolds was studied after three days by DAPI fluorescence microscopy. Furtheremore, microstructure observation with SEM suggests the formation of about 50 micrometer size porous structure and interconnected porosity so that cells adhesion within the structure is well in depth. Also, direct assay of cell proliferation performed with MTT test showed cells growing on the scaffold similar to or more than control samples result. Therefore, these findings suggest that the triblock-coated Poly (lactic-co-glycolic acid)/Hydroxyapatite porous composite scaffolds could provide cell adhesion and proliferation for tissue engineering

Tissue engineering; Porous scaffold; Freeze-extraction; Composite; humanMesenchymal stem cells (hMSC); Cell adhesion