Evaluating the effect of SPION-labeled amniotic membrane stem cell on heart failure

Introduction: Today, cell therapy is a common mean in the treatment of heart disease.So far, different types of stem cells have been used to enhance tissue repair in animal models of heart failure (HF) and myocardial infarction. However, stem cells, after injection, distribute to different tissues, which reduce the therapeutic effects of them.Objective: Evaluating the effect of amniotic membrane-derived stem cells on heart function, as well as increasing the implantation of cells containing iron oxide nanoparticles in heart with the aid of external magnetic field.Research Method: Thirty-five male rats weighing 180-200 g were divided into five groups: the control group, the group with heart failure, the group with heart failure, in which stem cells were intravenously injected, the group, in which stem cells containing iron oxide nanoparticles was intravenously injected and the last group that received stem cells containing iron oxide nanoparticles through the vein and also had external magnetic field on their heart. After 30 days, an echocardiography was performed, and then the iron levels in different tissues were measured using ICP.Results: The results showed that in the magnet dependent group, the intravenous injection of cells significantly increased the EF (63.70 - 2.7) and FS (30.18 - 1.65) compared to the HF group (21.92 - 1.69, 49.39 - 2.54), the cell injection group (24.15 - 0.86 and 54.13 - 1.10), and the magnet independent group (0.83 - 0.073). The iron levels of cells in the magnet dependent group (0.83 - 0.073) had also increased compared to the magnet independent group (0.46 - 0.078) (P<0.005).Discussion and conclusion: In fact, the results of this study, in line with conducted studies, showed that iron oxide nanoparticles can help in directing stem cells to specific points of the body that need repair and reconstruction using a magnetic field.

Keywords:

Amniotic membrane-derived stem cells , SPION , Magnetic field , Heart failure