PuraMatrix hydrogel enhances the expression of motor neuron progenitor marker and improves adhesion and proliferation of motor neuron-like cells

Publish Year: 1398
نوع سند: مقاله ژورنالی
زبان: English
View: 307

This Paper With 8 Page And PDF Format Ready To Download

  • Certificate
  • من نویسنده این مقاله هستم

استخراج به نرم افزارهای پژوهشی:

لینک ثابت به این Paper:

شناسه ملی سند علمی:

JR_IJBMS-23-4_003

تاریخ نمایه سازی: 27 مرداد 1399

Abstract:

Objective(s): Cell therapy has provided clinical applications to the treatment of motor neuron diseases. The current obstacle in stem cell therapy is to direct differentiation of stem cells into neurons in the neurodegenerative disorders. Biomaterial scaffolds can improve cell differentiation and are widely used in translational medicine and tissue engineering. The aim of this study was to compare the efficiency of two-dimensional with a three-dimensional culture system in their ability to generate functional motor neuron-like cells from adipose-derived stem cells. Materials and Methods: We compared motor neuron-like cells derived from rat adipose tissue in differentiation, adhesion, proliferation, and functional properties on two-dimensional with three-dimensional culture systems. Neural differentiation was analyzed by immunocytochemistry for immature (Islet1) and mature (HB9, ChAT, and synaptophysin) motor neuron markers. Results: Our results indicated that the three-dimensional environment exhibited an increase in the number of Islet1. In contrast, two-dimensional culture system resulted in more homeobox gene (HB9), Choline Acetyltransferase (ChAT), and synaptophysin positive cells. The results of this investigation showed that proliferation and adhesion of motor neuron-like cells significantly increased in three-dimensional compared with two-dimensional environments. Conclusion: The findings of this study suggested that three-dimension may create a proliferative niche for motor neuron-like cells. Overall, this study strengthens the idea that three-dimensional culture may mimic neural stem cell environment for neural tissue regeneration.

Authors

Marzieh Darvishi

Department of Anatomy, Faculty of Medicine, Ilam University of Medical Sciences, Ilam, Iran|Shefa Neuroscience Research Center, Khatam Alanbia Hospital, Tehran, Iran

Hatef Ghasemi Hamidabadi

Department of Anatomy & Cell Biology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran|Immunogenetic Research Center, Department of Anatomy & Cell Biology, Faculty of Medicine, Mazandaran University of Medical

Sajad Sahab Negah

Neuroscience Research Center, Mashhad University of Medical Sciences, Mashhad, Iran|Shefa Neuroscience Research Center, Khatam Alanbia Hospital, Tehran, Iran

Ardeshir Moayeri

Department of Anatomy, Faculty of Medicine, Ilam University of Medical Sciences, Ilam, Iran

مراجع و منابع این Paper:

لیست زیر مراجع و منابع استفاده شده در این Paper را نمایش می دهد. این مراجع به صورت کاملا ماشینی و بر اساس هوش مصنوعی استخراج شده اند و لذا ممکن است دارای اشکالاتی باشند که به مرور زمان دقت استخراج این محتوا افزایش می یابد. مراجعی که مقالات مربوط به آنها در سیویلیکا نمایه شده و پیدا شده اند، به خود Paper لینک شده اند :
  • Ross HH, Ambrosio F, Trumbower RD, Reier PJ, Behrman AL, ...
  • Pettikiriarachchi JT, Parish CL, Shoichet MS, Forsythe JS, Nisbet DR. ...
  • McNamara LE, McMurray RJ, Biggs MJ, Kantawong F, Oreffo RO, ...
  • Wichterle H, Lieberam I, Porter JA, Jessell TM. Directed differentiation ...
  • Dimos JT, Rodolfa KT, Niakan KK, Weisenthal LM, Mitsumoto H, ...
  • Thored P, Arvidsson A, Cacci E, Ahlenius H, Kallur T, ...
  • Darvishi M, Tiraihi T, Mesbah-Namin SA, Delshad A, Taheri T. ...
  • Negah SS, Khooei A, Samini F, Gorji A. Laminin-derived Ile-Lys-Val-ala-Val: ...
  • Cooke M, Vulic K, Shoichet M. Design of biomaterials to ...
  • Nakaji-Hirabayashi T, Kato K, Iwata H. In vivo study on ...
  • Loh QL, Choong C.Three-dimensional scaffolds for tissue engineering applications: role ...
  • Cavalcanti BN, Zeitlin BD, Nör JE. A hydrogel scaffold that ...
  • Raghavan S, Gilmont RR, Bitar KN. Neuroglial differentiation of adult ...
  • Cheng T-Y, Chen M-H, Chang W-H, Huang M-Y, Wang T-W. ...
  • Binan L, Tendey C, De Crescenzo G, El Ayoubi R, ...
  • Abdanipour A, Tiraihi T. Induction of adipose-derived stem cell into ...
  • Goutman SA, Chen KS, Feldman EL. Recent advances and the ...
  • Ghasemi-Kasman M, Hajikaram M, Baharvand H, Javan M. MicroRNA-mediated in ...
  • Miles GB, Yohn DC, Wichterle H, Jessell TM, Rafuse VF, ...
  • Alizadeh R, Kamrava SK, Bagher Z, Farhadi M, Falah M, ...
  • Alizadeh R, Hassanzadeh G, Joghataei MT, Soleimani M, Moradi F, ...
  • Negah SS, Khaksar Z, Aligholi H, Sadeghi SM, Mousavi SMM, ...
  • Frimat J-P, Xie S, Bastiaens A, Schurink B, Wolbers F, ...
  • Sun G, Liu W, Fan Z, Zhang D, Han Y, ...
  • Horii A, Wang X, Gelain F, Zhang S. Biological designer ...
  • Liqing Y, Jia G, Jiqing C, Ran G, Fei C, ...
  • Mohammadi Z, Afshari JT, Keramati MR, Alamdari DH, Ganjibakhsh M, ...
  • Gelain F, Bottai D, Vescovi A, Zhang S. Designer self-assembling ...
  • Tibbitt MW, Anseth KS. Dynamic microenvironments: the fourth dimension. Sci ...
  • Engler AJ, Sen S, Sweeney HL, Discher DE. Matrix elasticity ...
  • Thor S, Ericson J, Brännström T, Edlund T. The homeodomain ...
  • Pfaff SL, Mendelsohn M, Stewart CL, Edlund T, Jessell TM. ...
  • MacDonald SC, Fleetwood IG, Hochman S, Dodd JG, Cheng GK, ...
  • Naghdi M, Naghdi M, Tiraihi T, Namin SAM, Arabkheradmand J. ...
  • Adib S, Tiraihi T, Darvishi M, Taheri T, Kazemi H. ...
  • Liu N, Li Y, Yang S-T. Microfibrous carriers for integrated ...
  • Liu YQ, Zhan LB, Bi TT, Liang LN, Sun XX, ...
  • Sun Y, Li W, Wu X, Zhang N, Zhang Y, ...
  • نمایش کامل مراجع