Publisher of Iranian Journals and Conference Proceedings

Please waite ..
Publisher of Iranian Journals and Conference Proceedings
Login |Register |Help |عضویت کتابخانه ها
Paper
Title

Development of PCR-Based Method for Rapid Detection of Abrin Gene

فصلنامه گزارش های زیست فناوری کاربردی، دوره: 3، شماره: 3
Year: 1395
COI: JR_JABR-3-3_007
Language: EnglishView: 197
متن کامل این Paper منتشر نشده است و فقط به صورت چکیده یا چکیده مبسوط در پایگاه موجود می باشد.
توضیح: معمولا کلیه مقالاتی که کمتر از ۵ صفحه باشند در پایگاه سیویلیکا اصل Paper (فول تکست) محسوب نمی شوند و فقط کاربران عضو بدون کسر اعتبار می توانند فایل آنها را دریافت نمایند.

Buy and Download

متن کامل (فول تکست) این مقاله منتشر نشده و یا در سایت موجود نیست و امکان خرید آن فراهم نمی باشد.

Authors

Morteza Hamedi Behnaq - Molecular Biology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran
Ali Karami - Molecular Biology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran
Ali Choopani - Applied Biotechnology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran|Department of Biology, Payam Noor University, Isfahan, Iran

Abstract:

Abrin, known as a ribosome inactivating protein (RIP), is a high cytotoxic plant protein. The high lethality, low cost, and easy access to this plant and its seeds have led to this toxin to be used in crimes and terrorist acts. Since, obtaining purified toxins requires advanced laboratory equipment and complex procedures, it seems that the perpetrators of such crimes use crude extracts. As a result, it was hypothesized that remaining the specific toxin genes in these extracts can provide the advantage of using PCR assay to identify abrin gene which refers to the existence of its toxin. We used a new rapid molecular method for the detection of the abrin gene by PCR. In this regard, specific primers were designed and the required DNA was extracted from Rosary pie samples using cetyltrimethylammonium bromide-polyvinylpyrrolidone (CTAB-PVP) method and PCR protocol was performed using specific primers. Then, assay’s sensitivity was analyzed using serial dilution method. The results of this study revealed that designed and selected primers sequence for toxin’s gene function as specific. The desired product size was obtained and sequencing of PCR products showed up to 90% similarity with known sequence for each molecule. According to these results, the developed rapid molecular method for detection of abrin toxin gene can be considered as a sensitive and low-cost detection method for this toxin gene in cases of suspection to bioterrorism event.

Keywords:

Paper COI Code

This Paper COI Code is JR_JABR-3-3_007. Also You can use the following address to link to this article. This link is permanent and is used as an article registration confirmation in the Civilica reference:

https://civilica.com/doc/1043391/

How to Cite to This Paper:

If you want to refer to this Paper in your research work, you can simply use the following phrase in the resources section:
Hamedi Behnaq, Morteza and Karami, Ali and Choopani, Ali,1395,Development of PCR-Based Method for Rapid Detection of Abrin Gene,https://civilica.com/doc/1043391

Research Info Management

Certificate | Report | من نویسنده این مقاله هستم

اطلاعات استنادی این Paper را به نرم افزارهای مدیریت اطلاعات علمی و استنادی ارسال نمایید و در تحقیقات خود از آن استفاده نمایید.

Scientometrics

The specifications of the publisher center of this Paper are as follows:
Type of center: علوم پزشکی
Paper count: 1,602
In the scientometrics section of CIVILICA, you can see the scientific ranking of the Iranian academic and research centers based on the statistics of indexed articles.

Share this page

More information about COI

COI stands for "CIVILICA Object Identifier". COI is the unique code assigned to articles of Iranian conferences and journals when indexing on the CIVILICA citation database.

The COI is the national code of documents indexed in CIVILICA and is a unique and permanent code. it can always be cited and tracked and assumed as registration confirmation ID.

Support