A Review of the Methods for Concentrating M13 Phage

Publish Year: 1399
نوع سند: مقاله ژورنالی
زبان: English
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شناسه ملی سند علمی:

JR_JABR-7-1_002

تاریخ نمایه سازی: 16 شهریور 1399

Abstract:

Bacteriophages, viruses which infect the bacteria are the most abundant organisms on the earth. Among them, the best studied and the most-exploited group is the filamentous phages especially M13 phage. They have shown a lot of interesting applications because of their unique features. Therefore, to get maximum performance of bacteriophage like M13, techniques need to be engaged for proper concentrations. In this review, most of these methods were explored in PubMed, Scopus and Google Scholar, using keywords including M13 bacteriophage, phage concentration, phage purification, phage display. Accordingly, the most important research papers about this subject have been collected, categorized and discussed. As a conclusion, to select an appropriate method for the concentration of M13 bacteriophages different criteria should be considered, including cost, equipment, yield and purity of the product. In general, subsequent applications of M13 phage is the most important factor for the selection of the concentration method.

Authors

Faezeh Fouladvand

Department of Molecular Medicine and Genetic, Faculty of Medicine, Hamedan University of Medical Sciences, Hamedan, Iran

Peyman Bemani

Department of Immunology, Shiraz University of Medical Sciences, Shiraz, Iran

Mozafar Mohammadi

Applied Biotechnology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran

Razieh Amini

Department of Molecular Medicine and Genetic, Faculty of Medicine, Hamedan University of Medical Sciences, Hamedan, Iran