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Title

Investigation the Effect of Silver Nanoparticles and Bioresonance Wave Radiation on Leishmania major: An In Vitro Study

Investigation the Effect of Silver Nanoparticles and Bioresonance Wave Radiation on Leishmania major: An In Vitro Study
Year: 1399
COI: JR_JABR-7-1_008
Language: EnglishView: 278
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Authors

Nayereh Azimijou - Department of Biomedical Engineering, Amirkabir University of Technology, Tehran, Iran
Hamid Keshvari - Department of Biomedical Engineering, Amirkabir University of Technology, Tehran, Iran|Health Technology Research Institute, Amirkabir University of Technology, Tehran, Iran
Seyyed Javad Seyyed Tabaei - Department of Parasitology and Mycology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Mohsen Rahimi - Department of Parasitology and Mycology, School of Medicine, Student Research Committee, Shahid Beheshti University of Medical Sciences, Tehran, Iran|Department of Parasitology and Mycology, School of Medicine, Baqiyatallah University of Medical

Abstract:

Introduction: Cutaneous leishmaniasis (CL) is one of the infectious diseases and health problems in tropical regions. Glucantime is commonly used to treat CL and it, not only has some side effects but also observation shows the drug resistance of some of the various Leishmania species. Therefore, the aim of this study was to investigate the effect of silver nanoparticles (AgNPs) and bioresonance waves on Leishmania, in vitro. Materials and Methods: In the present experimental study, Leishmania major promastigotes were cultured in RPMI-1640 supplemented with 10% FBS and 1% penicillin and streptomycin at 23°C. After 6 days, the parasites achieved stationary phases of promastigotes, Then the effects of different concentrations of AgNPs (1, 3, 5, 10 and 25 μg/mL) and different radiation times of bioresonance wave (5 and 20 minutes) were investigated. Herein, the effects of various treatment on parasites proliferation were evaluated with live promastigotes counting after 24, 48 and 72 hours treatment. Results: The parasite count showed that the various concentrations of AgNPs, radiation of bioresonance wave and combination significantly decreased the numbers of live promastigotes over time compared with the control group after 72 hours. The highest antileishmanial activity was seen for AgNPs at concentration of 1 μg/mL when combined with 20 minutes radiation of bioresonance wave (proliferation inhibition: 79.92%). Conclusions: Based on our result, AgNPs and bioresonance waves are potent antileishmanial agents. The authors declare that the more studies should be done.

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Azimijou, Nayereh and Keshvari, Hamid and Seyyed Tabaei, Seyyed Javad and Rahimi, Mohsen,1399,Investigation the Effect of Silver Nanoparticles and Bioresonance Wave Radiation on Leishmania major: An In Vitro Study,https://civilica.com/doc/1043495

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Paper count: 22,251
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