Sima Orouei - MSc. Student, Department of Genetic Science, Medical Science Branch, Islamic Azad University, Tehran, Iran
Sedigheh Saberifar - Department of Basic Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
Hossein Bordbar - Histomorphometry and stereology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran- Department of Anatomical sciences, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
Ebrahim Rahmani Moghadam - Department of Anatomical sciences, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran

Introduction & Objectives: Colon cancer is the third most common cancer in the world. In recent years due to the increased prevalence of deaths from cancer and failure of chemotherapy in advanced forms of cancer, there is a need to find new ways to control cancer. One of these methods is the use of herbs. The aim of this study was to investigate the effect of cytotoxicity and autophagy of Glaucium flavum in HT-29 cell line and Beclin1 gene expression analysis.Material and Methods: In this study, the cytotoxicity of the Glaucium flavum extract was evaluated by MTT at concentrations of 25, 50, 100, and 200 μg/ml, and the concentration of 50 μg/ml was used for the determination of IC50. After treatment with IC50, cDNA was synthesized from extracted RNA. The expression of Beclin1 gene relative to the GAPDH gene was evaluated by QRT-PCR. The statistical analysis carried out using SPSS software.Results: The results showed that the concentration of 50 (μg / ml) of Glaucium flavum extract decreased cell viability, and also Beclin1 gene expression in HT-29 cells induces autophagy.Conclusion: Given the cytotoxicity of this extract, and the ability to induce autophagy process in HT-29 cell line, it can be concluded that this extract could be a viable candidate for cancer research.

Glaucium flavum, Beclin1, HT-29, Autophagy