Manijeh Rostaminia - Department of Agronomy, Shahr- e- Qods Branch, Islamic Azad University, Tehran, Iran
Davood Habibi - Department of Agronomy, Karaj Branch, Islamic Azad University, Karaj, Iran
Samira shahbazi - Nuclear Agriculture Research School, Nuclear Science and Technology Research Institute (NSTRI), Alborz, Iran.
Behzad Sani - Department of Agronomy, Shahr- e- Qods Branch, Islamic Azad University, Tehran, Iran
Alireza Pazoki - Department of Agronomy, Yadegar-e-Imam Khomeini (RAH) Shahr-e- Rey Branch, Islamic Azad University, Tehran, Iran.

Pseudomonas infections are an important cause of morbidity and mortality and saprophytic fungi are now increasingly being recognized as serious pathogens in immunocompromised patients.To investigate the effect of using bio-materials on mammalian tissues,two experiments were designed;the first one was feeding of Balb/c mice with irrigated lettuce with bio-fungicide (mutant and wild)and bio-fertilizers prepared with Pseudomonas (p) fluorescens, p. putida, p. aeruginosa, and the second was the usage of drinking water containing (Trichoderma (T) spores (mutant and wild) or P.fluorescens, P.putida, P.aeruginosa suspensions). Then, blood factors and inflammation of tissues (liver, kidney, spleen and large intestine) in all mice were analyzed after two months. Blood samples were taken from the mice to examine some of the hematological (RBC, MCV, MCH, MCHC) (data not shown) and biochemical (AST, ALT, ALP) factors, and also observed under a microscope. The study of tumor marker carcinoembryonic antigen (CEA) in all treatments showed that the strains in these bio-fertilizers did not stimulate carcinogenic indices.The results from the other blood factors were normal for all strains (data not shown).Only P.putida showed no adverse effect on the increase in alkaline phosphatase (ALP).The results also showed that the effect of bio-fungicide on mammalian tissues (spleen and large intestine) was normal. But a small number of mild liver necrosis was seen in the treatment groups with wild Trichoderma, and moderate necrosis in the the liver tissue after treatment with mutant Trichoderma isolates.More investigation should be made to determine the impact of these biotic factors on the mammalian tissues before commercialization.

Pseudomonas, Fertilizer, Biological Fungicide, Trichoderma