C-MAF Expression in Adult Human Ocular Surface and its Implication in Pterygium Pathogenesis
Publish Year: 1398
نوع سند: مقاله ژورنالی
زبان: English
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شناسه ملی سند علمی:
JR_RBMB-8-4_010
تاریخ نمایه سازی: 22 دی 1399
Abstract:
Background: c-MAF, a transcription factor that belongs to the b-Zip Maf transcription factor family, was found to be critical for lens development in vertebrates. It is a well-known fact that the adult human ocular surface expresses c-MAF, however, its role in the limbus, cornea and conjunctiva remains unknown. Thus, the present study aimed to investigate c-MAF expression within the human ocular surface, and its potential role in pterygium pathogenesis.
Methods: We performed immunohistochemical staining to detect c-MAF expression in frozen adult human tissue sections, including the limbus, cornea and conjunctiva, and cultured cells from eye cadavers. We then compared c-MAF expression to the expression of a known protein, P63. Lastly, we performed RT-PCR, and immunohistochemistry for c-MAF expression in healthy adult human conjunctiva and pterygium.
Results: We found differential c-MAF expression between adult human limbus, cornea and conjunctiva tissues. Further, we observed that c-MAF is downregulated in the pterygium compared to healthy conjunctiva.
Conclusions: Overall, our results suggest that c-MAF may play a context-specific role in maintaining limbal, corneal and conjunctival homeostasis, and may be critical for preventing pterygium development in humans.
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Authors
Hasan Mahmud Reza
Department of Pharmaceutical Sciences, North South University, Dhaka ۱۲۲۹, Bangladesh.
Razwa Saleh
Department of Pharmaceutical Sciences, North South University, Dhaka ۱۲۲۹, Bangladesh.
Preeti Jain
Department of Pharmaceutical Sciences, North South University, Dhaka ۱۲۲۹, Bangladesh.
Ghazi Muhammad Sayedur Rahman
Department of Pharmaceutical Sciences, North South University, Dhaka ۱۲۲۹, Bangladesh.
Asim Kumar Bepari
Department of Pharmaceutical Sciences, North South University, Dhaka ۱۲۲۹, Bangladesh.