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Title

Introducing the Best Six Loci in Mycobacterial Interspersed Repetitive Unit-Variable-Number Tandem Repeat (MIRU-VNTR) Typing for Mycobacterium TuberculosisGenotyping

مجله گزارش های بیوشیمی و زیست شناسی مولکولی، دوره: 8، شماره: 3
Year: 1398
COI: JR_RBMB-8-3_015
Language: EnglishView: 141
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Authors

Mahdis Ghavidel - Antimicrobial Resistance Research Center, Mashhad University of Medical Sciences, Mashhad, Iran. & Department of Microbiology and Virology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
Keyvan Tadayon - Department of Microbiology, Razi Vaccine and Serum Research Institute (RVSRI), Agricultural Research, Education and ExtensionOrganization (AREEO), Karaj, Iran
Nader Mosavari - PPD Tuberculin Department, Razi Vaccine and Serum Research Institute, (RVSRI), Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran.
Kimiya Nourian - Doctor of Veterinary Medicine, Graduate Student of School of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad,Iran.
Hamid Reza BahramiTaghanaki - School of Persian Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
Gholam Reza Mohammadi - Department of Clinical Sciences, School of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad –Iran.
Mohammad Rashtibaf - Deputy of Veterinary administration of Khorasan Razavi Province, Mashhad,Iran.
Kiarash Ghazvini - Antimicrobial Resistance Research Center, Mashhad University of Medical Sciences, Mashhad, Iran. & Department of Microbiology and Virology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.

Abstract:

Background: Tuberculosis (TB) still remains endemic worldwide making epidemiological studies essential to mitigating efforts implicated in identifying its source,controlling, and preventing the spread of dangerous strains amongst humans such as Mycobacterium tuberculosis (Mtb). Methods: In this study, we sought to determine the 6 Mycobacterial Interspersed Repetitive Unit-Variable-Number Tandem Repeat (MIRU-VNTR) loci with high discriminatory powers for Mtb genotypingas well as the loci with the highest and the lowest discriminatory powers for MIRU-VNTR.To conduct our search, we used several databases such as science direct, Embase (Elsevier), Web of Science, Scopus and Medline via PubMed. Searches were performed using key words including: Mycobacterium tuberculosis, MIRU–VNTR, Allele diversity, Genetic diversity and human patient. Finally, 56 articles were selected after filtering out titles, abstracts and full texts. Results: Loci with high discriminatory powers included MIRU10 and MIRU26, while MIRU2, MIRU20, MIRU24 and ETRD had poor discriminatory powers. According to previous data in the literature, the loci MIRU10, MIRU26, MIRU40, QUB 26, QUB 11b and Mtub21 have high discriminatory powers. Conclusions:Therefore, these loci recommended for genotyping Mtb to save time and cost and to ensure the production of reliable results.

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This Paper COI Code is JR_RBMB-8-3_015. Also You can use the following address to link to this article. This link is permanent and is used as an article registration confirmation in the Civilica reference:

https://civilica.com/doc/1141922/

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Ghavidel, Mahdis and Tadayon, Keyvan and Mosavari, Nader and Nourian, Kimiya and BahramiTaghanaki, Hamid Reza and Mohammadi, Gholam Reza and Rashtibaf, Mohammad and Ghazvini, Kiarash,1398,Introducing the Best Six Loci in Mycobacterial Interspersed Repetitive Unit-Variable-Number Tandem Repeat (MIRU-VNTR) Typing for Mycobacterium TuberculosisGenotyping,https://civilica.com/doc/1141922

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