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Title

A Simplified Protocol for the Purification of Schwann Cells and Exosome Isolation from C57BL/6 Mice

Year: 1397
COI: JR_RBMB-7-1_002
Language: EnglishView: 92
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Authors

Mana Shojapour - Department of molecular Medicine. School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Ghasem Mosayebi - Molecular and Medicine Research Center, Arak University of Medical Sciences, Arak, Iran.
Reza Hajihossein - Department of Parasitology, Arak University of Medical Sciences, Arak, Iran. Department of Immunology, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Farshid Noorbakhsh - Department of Immunology, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran
Aram Mokarizadeh - Department of Immunology, Faculty of Medicine, Cellular and Molecular Research Center, Kurdistan University of Medical Sciences, Sanandaj, Iran.
Mohammad Hossein Ghahremani - Department of Molecular Medicine, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran.

Abstract:

Background: The purification of Schwann cells has proven to be a difficult process, with most methods requiring the use of special equipment. However, obtaining a sufficient number and high purity of Schwann cells is an integral aspect in their use for clinical application. Therefore, the aim of this study was to establish a simple and effective protocol for the isolation and purification of Schwann cells from the sciatic nerve of C57BL/6 mice. Furthermore, we aimed to provide a protocol for the isolation of exosomes from these cells. Methods: To purify Schwann cells, we used a combination of in situ nerve pre-degeneration and fetal bovine serum. To determine the most effective method of cell purification, we treated the culture with varying concentrations of fetal bovine serum and examined which concentration provided the highest Schwann cell purity. Exosomes were then isolated from Schwann cells through a process of repeated centrifugation and filtration steps. Results: We were able to increase the purified population of Schwann cells from C57BL/6 mice by reducing the concentration of FBS. The purity of Schwann cells at FBS concentrations of 10%, 5%, and 2% were 93.42%, 91.25%, and 97.83%, respectively. Conclusions: When using a concentration of 2% FBS, we obtained the highest purification yield of Schwann cells. Our protocol does not require special equipment or materials. We have created a protocol that is simple, fast, and safe while providing a high yield of purified Schwann cells. The exosome isolation method described in this paper is an appropriate approach with a high quality and yield.

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This Paper COI Code is JR_RBMB-7-1_002. Also You can use the following address to link to this article. This link is permanent and is used as an article registration confirmation in the Civilica reference:

https://civilica.com/doc/1141969/

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If you want to refer to this Paper in your research work, you can simply use the following phrase in the resources section:
Shojapour, Mana and Mosayebi, Ghasem and Hajihossein, Reza and Noorbakhsh, Farshid and Mokarizadeh, Aram and Ghahremani, Mohammad Hossein,1397,A Simplified Protocol for the Purification of Schwann Cells and Exosome Isolation from C57BL/6 Mice,https://civilica.com/doc/1141969

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Type of center: علوم پزشکی
Paper count: 8,531
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