Construction of a Novel DNA Vaccine Candidate encoding LmSTI1-PpSP42 Fusion Protein from Leishmania major and Phlebotomus papatasi against Cutaneous Leishmaniasis
عنوان مقاله: Construction of a Novel DNA Vaccine Candidate encoding LmSTI1-PpSP42 Fusion Protein from Leishmania major and Phlebotomus papatasi against Cutaneous Leishmaniasis
شناسه ملی مقاله: JR_RBMB-7-1_010
منتشر شده در در سال 1397
شناسه ملی مقاله: JR_RBMB-7-1_010
منتشر شده در در سال 1397
مشخصات نویسندگان مقاله:
Touraj Miandoabi - Department of Parasitology and Mycology, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Fariborz Bahrami - Pasteur Institute of Iran, Department of Immunology, ۶۹ Pasteur Ave., Tehran, Iran.
Vahideh Moein Vaziri - Department of Parasitology and Mycology, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Soheila Ajdary - Pasteur Institute of Iran, Department of Immunology, ۶۹ Pasteur Ave., Tehran, Iran.
خلاصه مقاله:
Touraj Miandoabi - Department of Parasitology and Mycology, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Fariborz Bahrami - Pasteur Institute of Iran, Department of Immunology, ۶۹ Pasteur Ave., Tehran, Iran.
Vahideh Moein Vaziri - Department of Parasitology and Mycology, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Soheila Ajdary - Pasteur Institute of Iran, Department of Immunology, ۶۹ Pasteur Ave., Tehran, Iran.
Background: Cutaneous leishmaniasis (CL) is a serious public health problem in many tropical countries. The infection is caused by a protozoan parasite of Leishmania genus transmitted by Phlebotominae sandflies. In the present study, we constructed a eukaryotic expression vector to produce a fusion protein containing LmSTI1 from Leishmania major (L. major) and PpSP42 from Phlebotomus papatasi (Ph. papatasi). In future studies we will test this construct as a DNA vaccine against zoonotic CL.
Methods: The nucleotide sequences encoding the LmSTI1 protein and a fragment encoding 79% of PpSP42 were amplified using L. major and Ph. papatasi genomic DNA, respectively. The amplicons were cloned into the pcDNA3.1(+) eukaryotic expression vector. The recombinant plasmid pcDNA-LmSTI1Pp42 was propagated in Escherichia coli (E. coli) and used to transfect HEK-293T cells. The expressed fusion protein was analyzed by Western blotting using anti-LmSTI1 mouse serum.
Results: Sequences encoding LmSTI1 and partial PpSP42 were cloned into pcDNA3.1(+). Production of the recombinant LmSTI1Pp42 fusion protein was confirmed by Western blotting.
Conclusions: An LmSTI1Pp42 fusion protein was expressed HEK-293T cells. This construct may be an effective DNA vaccine against CL.
کلمات کلیدی: Cloning, DNA vaccine, Leishmania major, LmSTI1, PpSP42.
صفحه اختصاصی مقاله و دریافت فایل کامل: https://civilica.com/doc/1141977/