Promoter analysis of the AP2/ERF domain-containing protein gene in barley(Hordeumvulgare L.)unravelsits putativeinvolvement in abiotic stress responses

One of the biggest families that play a role in growth and development is AP2/ERF. It also plays a role in hormone responses and biotic/abiotic stress responses in plants.HORVU6Hr1G075640 encodes AP2/ERF domain-containing protein in barley.However, information on HORVU6Hr1G075640 remains lacking.Genomic data analysis can lead to deeper knowledge of the functional role of a gene. So, in this study, promoter motifs were surveyed via motifbased sequence analysis tools such as MEME (http://meme-suite.org/tools/meme), GOMO (http://memesuite. org/tools/gomo), FIMO (http://meme-suite.org/tools/fimo). The results of this study indicate 38 significantmotif sequences with lengths ranging from 8 to 50bp in the promoter regions.Motif 4, motif 16, 19, and 10 have the most occurrences. Moreover, the majority of motifs have a relationship with AP2EREBP family, 21.05%.Motifs were related to biological processes such as regulation of transcription, transmembrane receptor protein tyrosinekinase signaling pathway, and translation.With regard to the promoter analysis applied in this study, it can be interpreted that HORVU6Hr1G075640 might be closely related with abiotic stress and have potential functions in the abiotic stress tolerance. The results from this study will serve as a basis for the functional analyses of HORVU6Hr1G075640 in barley.

Keywords:

Barley , Gene Ontology , Promoter analysis