Comparison of a Nucleic Acid Sequence-based Amplification (NASBA) and real-time reverse transcriptase PCR methods for detection of Toxoplasma gondii in rat blood samples

Publish Year: 1395
نوع سند: مقاله ژورنالی
زبان: English
View: 205

This Paper With 9 Page And PDF Format Ready To Download

  • Certificate
  • من نویسنده این مقاله هستم

استخراج به نرم افزارهای پژوهشی:

لینک ثابت به این Paper:

شناسه ملی سند علمی:

JR_JZD-1-1_002

تاریخ نمایه سازی: 27 بهمن 1399

Abstract:

The numbers of RNA amplification methods for detection of Toxoplasma spp. are increasing, however comparative studies on the performance of these different assays are lacking. The aim of this study was to compare two molecular assays for detection and quantification of Toxoplasma spp. in blood samples collected from experimentally infected rats. A set of specific primers and beacon probe were selected from the B1 rRNA gene of Toxoplasma. The assays using real-time detection proved to be both sensitive and specific. Nucleic acid sequence-based amplification (NASBA) method for detection of Toxoplasma spp. have advantages regarding sensitivity and potential quantitative population dynamics of Toxoplasma gondii in comparison with the RT-PCR method, but it is not often routinely used at present. NASBA had a detection limit of 1 parasite/ml of blood, while RT-PCR detected 10 parasites/ml. The results of real-time NASBA can be obtained 12h earlier. Therefore, sooner than the ordinary real-time RT-PCR the use of real-time NASBA is preferred to the ordinary real-time RT-PCR.

Authors

- -

Department of Pathobiology, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran

- -

Department of Parasitology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran

- -

Department of Biotechnology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran

- -

Department of Parasitology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran

مراجع و منابع این Paper:

لیست زیر مراجع و منابع استفاده شده در این Paper را نمایش می دهد. این مراجع به صورت کاملا ماشینی و بر اساس هوش مصنوعی استخراج شده اند و لذا ممکن است دارای اشکالاتی باشند که به مرور زمان دقت استخراج این محتوا افزایش می یابد. مراجعی که مقالات مربوط به آنها در سیویلیکا نمایه شده و پیدا شده اند، به خود Paper لینک شده اند :
  • Adriana C., Giovanna P., Chiara G., Simona P., Laura Z., ...
  • Bretagne S., Costa JM., Vidaud M., Tran J., Nhieu V. ...
  • Boyer K.M., Holfels E., Roizen N., Swisher C., Mack D., ...
  • Costa J.M., Pautas C., Ernault P., Foulet F., Cordonnier C. ...
  • Duval X., Pajot O., Le Moing V., Longuet P., Ecobichon ...
  • Echevarria J., Erdman D.D., Swierkosz E.M., Holloway B.P., and Anderson ...
  • Klein P.G. and Juneja V.K. (1997). Sensitive detection of viable ...
  • Kievits T., Vangemen B., Vanstrijp D., Schukkink R., Dircks M., ...
  • Leone G., van Schijndel H., Van Gemen B., Kramer F.R. ...
  • Montoya J.G. and Liesenfeld O. (2004). Toxoplasmosis. Lancet, 363, pp.1965–1976. ...
  • Meide Van der W.F., Schoone GJ., Faber W.R., Zeegelaar J.E., ...
  •  Mugasa C.M., Schoone G.J., Rosine A.E., Lubega G.W., Kager P.A. ...
  • Sheridan G.E.C., Masters C.I., Shallcross J.A. and MacKey B.M. (1998). ...
  • Schoone G.J., Oskam L., Kroon N.C.M., Schallig H.D.F.H. and Omar ...
  • Stanway G. (1990). Structure, function and evolution of picornaviruses. Journal ...
  • Tyagi S. and Kramer F.R. (1996). Molecular beacons: probes that ...
  • نمایش کامل مراجع