Fatemeh Kianinodeh - High Institution for Research and Education in Transfusion Medicine, Tehran, Iran
Mahdi Tabatabaei - Department of Laboratory Sciences, College of Science of Borujerd, Islamic Azad University, Iran and Young Researchers and Elite Club, Borujerd Branch, Islamic Azad University, Borujerd, Iran
Atena Alibakhshi - Department of Laboratory Sciences, College of Science of Borujerd, Islamic Azad University, Iran
Mahshid Gohari - Department of Laboratory Sciences, College of Science of Borujerd, Islamic Azad University, Iran
Kaveh Tari - Cancer and Immunology Center, Kurdistan University of Medical Sciences Sanandaj, Iran

Introduction: Breast cancer is one of the most common malignancies among Iranian women. Nowadays, the use of traditional medicinal plants has emerged as a tempting complementary to the treatment of breast cancer due to minimal side effects and less documented drug resistance. The aim of this study was to examine the anticancer effects of the essential oils of red clover and mature fruit of Bassia scoparia on MCF-7 cells. Methods: Essential oils were extracted from Bassia scoparia and red clover plants, by a Clevenger condenser and mass spectrometry was performed for qualitative analysis, then MCF-7 and HU02 cells were treated with different concentrations of these essential oils at different time intervals. The viability of cells was measured by the MTT assay. The results were analyzed using the one-way ANOVA and Tukey′s test. Results: Essential oils of Kochia scoparia and red clover have cytotoxic effects on MCF7 cells. The MTT analysis showed decreased cell viability percentage in treated cells (P <0.05). In addition, the microscopic examination of cells treated with essential oils revealed morphological changes of apoptotic cells in MCF-7 cells. Conclusions: The findings of this study show that essential oils of Bassia scoparia and red clover have toxic and anticancer effects on MCF-7 cells.

Bassia Scoparia, Red Clover, Cell Viability.