M Farhadian - Isfahan High Educationand Research Institute, P.O.Box ۸۳۱۴۵-۴۱۶, Isfahan, Iran
A Zinatizadeh - Applied chemistry department, Faculty of chemistry, Razi University, Kermanshah, Iran
M Borghei - BBRC, Sharif University of Technology, Tehran, Iran
D Duchez - Laboratoire de Génie Chimique et Biochimique, Polytech’ Clermont-Ferrand, Université Blaise Pascal, France

In this approach, toluene remediation from contaminated water in two steps containing solute adsorption onto granular activated charcoal (GAC) and solids waste regeneration in anaerobic bioreactor was investigated. Data showed that solute adsorption by GAC in closed system is highly successful in removing of toluene compound till standard water levels. This approach investigated that GAC particle as hazardous wastes, after loading with toluene hydrocarbon, can be regenerated by Thauera aromatica K172 in closed anaerobic bioreactor with nitrate respiration. Data highlighted the fact that 90% of contaminant onto GAC (100 mg toluene/ g GAC) can be biodegraded in batch unit, after 2 days. Also, the overall stoichiometry of toluene utilization onto GAC by biomass to nitrate reduction was 1: 6.2.

Monoaromatic Hydrocarbon, Toluene, Bioregeneration, Anaerobic Bioreactor, Biphasic System