Fabrication of co-delivery systems based on graphene-g-cyclodextrin/ chitosan nanofiber

Nowadays, co-loading of therapeutic drugs into one drug delivery system has revolutionized in the medical field to improve the therapeutic activity.1-3 Combination therapy provides an effective strategy to achieve high therapeutic concentration compared with single drug therapy, In this regard, polymer nanocarriers with core–shell nanostructures are the most prevalent carrier in these applications.4 Here, we designed co-loaded delivery systems, composed of curcumin loaded cyclodextrin-graphene oxide core (Cur@CD-GO) and gallic acid loaded chitosan shell (Ga@Cs) nanofibers (Cur-Ga NF), which can promote the therapeutic efficiency of drugs. The synthesized nanofibres were fabricated by electrospinning technique with the coaxial system. It was demonstrated that the nanofibers were successfully prepared, and the drugs in the core and shell of nanofibers were released in a controlled and sustained manner. MTT assay, antibacterial activity, antioxidant activity, and the anti-inflammatory assay were used for evaluation the performance of nanofibers. The produced Cur-Ga NF, providing improved anti-cancer activity, antimicrobial activity, antioxidant activity and anti-inflammatory outcome as compared to single drug-loaded NFs (Cur@CD-GO NF). Our investigations showed that such co-delivery fiber systems could be employed as a promising nanocarrier for therapeutic applications.

Keywords:

Nanofiber , Graphene oxide , Chitosan , Curcumin and Gallic acid