Abbas Asoudeh Fard - INSERM U۱۱۴۸, Laboratory for Vascular Translation Science (LVTS), Cardiovascular Bioengineering, University Sorbonne Paris North, Paris, France. Research Center for Pharmaceutical Nanotechnology, Tabriz University of Medical Sciences, Tabriz, Iran

Astaxanthin is one of famous carotenoid showing efficient scavenging ability and signifies an interesting candidate in the progress of new therapies for stopping and treating oxidative stress-related pathologies. So for study effect astaxanthin in the cell we need to make stress condition in the cells and forthis purpose, used AMA and AAPH. Method: We cultured HUVEC cells and ready it for MTT assay. For this purpose, treatment by different concentrationof AAPH (80 mM- 10 mM) and AMA (75 μM-12.5 μM) for find the best IC50 of AMA and AAPH. For study gene expression, treatment astaxanthin were studied HO-1, NQO-1, Nrf2 and SOD1 mRNA expression was analyzed using quantitative real-time PCR.Results: Our findings showed that the best concentration IC50of AMA is 12.5 μM and for AAPH is 10 mM, and we showed astaxanthin could affect the signal transduction through HO-1, NQO-1, Nrf2, and SOD1 and protect cell

Astaxanthin, Oxidative Stress, Apoptosis.