Evaluation of the effect of somatic antigens of Salmonella on the expression levels of CD40 and CD86 molecules in antigen presenting cells

Salmonella is a gram-negative bacillary bacterium that causes important diseases such as gastroenteritis and septicaemia in humans. Adaptive immuneresponses are crucial in the control of Salmonella infection in the body. Antigen presenting cells are important players in the triggering of adaptive immuneresponses and their maturation is necessary for induction of adaptive immune responses. There is not enough information on the effects of Salmonellaantigens in the induction of maturation in antigen presenting cells. The aim of this study was evaluation of the effect of somatic antigens of the gram-negativebacterium on induction of maturation in antigen presenting cells in vitro. Antigen presenting cells were differentiated from bone marrow precursor cellsin the presence of Granulocyte-Macrophage colony stimulating factor in culture medium. The differentiated antigen presenting cells were exposed to bacterial somatic antigens for 24 h. Then, expression levels of costimulatory molecules CD40 and CD86 were measured in antigen presenting cells. Data analysis showed that the expression levels of CD40 and CD86 were low in in vitrodifferentiated antigen presenting cells. The expression levels of thesecostimulatory molecules were significantly higher in antigen presenting cells cultured in the presence of bacterial somatic antigens than that in controlantigen presenting cells. These findings indicate that bacterial somatic antigens can induce maturation of antigen presenting cells.

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Department of laboratory sciences , School of Allied Medical Sciences , Tehran University of Medical Sciences , Tehran , Iran