Sara Darbandi - Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran.
Mahsa Darbandi - Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran.
Ashok Agarwal - American Center for Reproductive Medicine, Cleveland Clinic, Cleveland, Ohio, ۴۴۱۹۵, USA.
Hamid Reza Khorram Khorshid - Genetics Research Center, University of Social Welfare and Rehabilitation Sciences, Tehran, Iran.
Mohammad Reza Sadeghi - Department of Embryology and Andrology, Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran
Sandro C.Esteves - Androfert, Andrology and Human Reproduction Clinic, Campinas, Brazil.
Pallav Sengupta - Department of Physiology, Faculty of Medicine, Mahsa University, Selangor, Malaysia.
Sulagna Dutta - Department of Physiology, Faculty of Medicine, Mahsa University, Selangor, Malaysia.
Zohreh Fathi - Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran.
Hojjat Zeraati - Department of Epidemiology and Biostatistics, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
Mohammad Mehdi Akhondi - Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran.

Background: The three-parent assisted reproductive technique may increase oocyte competence. Objective: In this case-control study, the suitability of germinal vesicle transfer (GVT), synchronous ooplasmic transfer (sOT), asynchronous ooplasmic transfer using cryopreserved MII oocyte (caOT), and asynchronous ooplasmic transfer using waste MII oocyte (waOT) for maturation of the human-aged non-surrounded nucleolus germinal vesicle-stage (NSN-GV) oocyte were investigated. Materials and Methods: NSN-GV oocytes were subjected to four methods: group A (GVT), B (sOT), C (caOT) D (waOT), and E (Control). The fusion rates, MI, MII, ICSI observations and cleavage at 2-cell, 4-cell, and 8-cell stages were compared in the groups. Results: In GVT, none of the oocytes fused. In sOT, all oocytes fused, 20 achieved the MI, 14 progressed to MII, 8 fertilized, 6 cleaved and 5, 4, and 3 achieved the 2-cells, 4-cells and 8-cells, respectively. In caOT, all oocytes fused and achieved the MI, 8 progressed to MII and fertilized, 6 cleaved and 6, 5, and 5 achieved the 2-cells, 4-cells, and 8-cells respectively. In waOT, all oocytes fused, 5 and 3 progressed to MI and MII, respectively, but only one fertilized, cleaved and reached a 4-cells stage. In group E, 6 and 2 oocytes progressed to MI and MII, respectively, and only one fertilized but arrested at the zygote stage. caOT had the highest survival rate when compared to sOT (p = 0.04), waOT (p = 0.002), and control (p = 0.001). Conclusion: The caOT method was beneficial over sOT, waOT, and GVT in supplementing the developmental capacity of human-aged NSN-GV oocytes.

Assisted reproductive techniques, In vitro oocyte maturation techniques, Nuclear transfer techniques, Oocytes, Oocyte donation., تکنیک های کمک باروری, تکنیک بلوغ آزمایشگاهی تخمک, تکنیک انتقال هسته, تخمک, اهدای تخمک.