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Title

The Comparative Effect of Nisin and Thioridazine as Potential Anticancer Agents on Hepatocellular Carcinoma

مجله گزارش های بیوشیمی و زیست شناسی مولکولی، دوره: 9، شماره: 4
Year: 1399
COI: JR_RBMB-9-4_011
Language: EnglishView: 110
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Authors

Noha El-Sayed Ibrahim - Department of Microbial Biotechnology, Genetic Engineering and Biotechnology Division, National Research Centre, Dokki, Giza, Egypt.
Heba Morsy - Department of Medical Biochemistry & Molecular Biology, Faculty of Medicine, Cairo University.
Marwa Abdelgwad - Department of Medical Biochemistry & Molecular Biology, Faculty of Medicine, Cairo University.

Abstract:

Abstract: Background: Hepatocellular carcinoma is a major health problem worldwide especially in Egypt. It accounts for the fifth common cancer and the second cause of death among different cancers. This study investigated the efficacy and molecular mechanism of Nisin and/or Thioridazine as anticancer treatment on human liver cancer HepG2 cell line. Methods: Nisin and Thioridazine were applied for 24 h on human liver cancer cell line (HepG2). 3-(4, 5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was done to assess the cytotoxicity of Nisin and Thioridazine. Quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) was used for the assessment of PI3K, AKT, SIRT-1, and NRF2 expression in the treated cell line. The protein level of reactive oxygen species (ROS) and vascular endothelial growth factor (VEGF) was measured in the collected media by ELISA technique. Western blot analysis was done for, tAKT, pAKT, tPI3K, and pPI3K. Results: Cell proliferation results showed that compared with the untreated cancer, Nisin and/or Thioridazine treated groups had decreased cell proliferation (p value< 0.0001). Nisin and/or Thioridazine decreased PI3K/AKT mRNA and protein expression in hepatocellular carcinoma cells (HCC). Also Nisin and/or Thioridazine decreased anti-oxidative SIRT1/NRF2 mRNA expression. ROS level highly increased with Nisin and/or Thioridazine treatment in contrast to VEGF protein level which was highly decreased. Conclusions: These results introduce Nisin and Thioridazine as new therapeutic lines in HCC.

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Paper COI Code

This Paper COI Code is JR_RBMB-9-4_011. Also You can use the following address to link to this article. This link is permanent and is used as an article registration confirmation in the Civilica reference:

https://civilica.com/doc/1177955/

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If you want to refer to this Paper in your research work, you can simply use the following phrase in the resources section:
Ibrahim, Noha El-Sayed and Morsy, Heba and Abdelgwad, Marwa,1399,The Comparative Effect of Nisin and Thioridazine as Potential Anticancer Agents on Hepatocellular Carcinoma,https://civilica.com/doc/1177955

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