Voltage-gated potassium channels are involved in oxymatrine-regulated islet function in rat islet β cells and INS-۱ cells
Publish place: Iranian Journal of Basic Medical Sciences، Vol: 24، Issue: 4
Publish Year: 1400
نوع سند: مقاله ژورنالی
زبان: English
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شناسه ملی سند علمی:
JR_IJBMS-24-4_006
تاریخ نمایه سازی: 6 اردیبهشت 1400
Abstract:
Objective(s): Oxymatrine can regulate glucose metabolism. But the underlying mechanisms remain unclear. We investigated the relationship of oxymatrine and voltage-gated potassium (Kv) channel in rat islet β cells and INS-۱ cells.Materials and Methods: Insulin secretion and Kv channel currents were tested by radioimmunoassay and patch-clamp technique, respectively. The INS-۱ cell viability was detected using cell counting kit-۸ experiments. Flowcytometry analysis and western blot were employed for cell apoptosis and protein levels, respectively. INS-۱ cell proliferation was assessed by the ۵-Ethynyl-۲’- deoxyuridine method. Results: Oxymatrine potentiated insulin secretion at high glucose (p Conclusion: The results indicate that oxymatrine can stimulate insulin secretion and decrease kv channel currents in islet β cells. Besides, oxymatrine also increases cell viability, proliferation, and reduces cell apoptosis in INS-۱ cells. The effects of oxymatrine are related to kv channels. This finding provides new insight into the mechanisms of oxymatrine-regulated islet function.
Keywords:
Apoptosis Diabetes mellitus Insulin secretion Oxymatrine Potassium channel Voltage , gated
Authors
Jingying Gao
Department of Pediatrics, Shanxi Medical University, Taiyuan, China
Lixia xia
Department of Pediatrics, Shanxi Medical University, Taiyuan, China
Yuanyuan Wei
Department of Pediatrics, Shanxi Medical University, Taiyuan, China
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