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Title

Pathogenic role of the SP/ NK۱R system in GBM cells through inhibiting the thioredoxin system

Pathogenic role of the SP/ NK۱R system in GBM cells through inhibiting the thioredoxin system
Year: 1400
COI: JR_IJBMS-24-4_011
Language: EnglishView: 142
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Authors

Fatemeh Ghahremani - Department of Biology, Faculty of Science, Hakim Sabzevari University, Sabzevar, Iran
Reihaneh Sabbaghzade - Department of Biology, Faculty of Science, Hakim Sabzevari University, Sabzevar, Iran
Safieh Ebrahimi - Department of Clinical Biochemistry, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
Hosein Javid - Medical Laboratory Sciences Department, Varastegan Institute for Medical Sciences, Mashhad, Iran
Javad Ghahremani - Department of Medicine, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Seyed Isaac Hashemy - Department of Clinical Biochemistry, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

Abstract:

Objective(s): Glioblastoma multiforme (GBM), a highly aggressive Grade IV brain tumor, is a significant public health issue due to its poor prognosis and incurability. Neuropeptide substance P (SP) plays a critical role in GBM tumor growth and development via activation of neurokinin‐۱receptor (NK۱R). Moreover, SP is a pro-oxidant factor contributing to oxidative stress in various cell types. However, the link between SP and oxidative stress in cancer cells is not fully investigated. Here, we aimed to identify the effects of SP and NK۱R antagonist, aprepitant, on the redox status of GBM cells.Materials and Methods: Resazurin assay was employed to determine the effect of aprepitant on viability of U۸۷ glioblastoma cells. ۲’,۷’-dichlorodihydrofluorescein diacetate (H۲DCFDA) assay was employed to measure the levels of intracellular reactive oxygen species (ROS). A quantitative real-time polymerase chain reaction was applied to measure the expression of proteins of the thioredoxin system. Commercial kits (ZellBio GmbH) were also used to measure the enzymatic activity of these proteins.Results: We found that SP increased ROS level in U۸۷ GBM cells, and aprepitant significantly reduced this effect. Furthermore, we found that SP could also affect the thioredoxin system, a central antioxidant enzyme defense system. SP reduced both expression and enzymatic activity of the thioredoxin system’s proteins, Trx and thioredoxin reductase (TrxR) and these effects were significantly reduced by aprepitant. Conclusion: Our results indicated that SP activation of NK۱R represented a link between oxidative stress and GBM and highlighted the need for further validations in future studies.

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This Paper COI Code is JR_IJBMS-24-4_011. Also You can use the following address to link to this article. This link is permanent and is used as an article registration confirmation in the Civilica reference:

https://civilica.com/doc/1186920/

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Ghahremani, Fatemeh and Sabbaghzade, Reihaneh and Ebrahimi, Safieh and Javid, Hosein and Ghahremani, Javad and Hashemy, Seyed Isaac,1400,Pathogenic role of the SP/ NK۱R system in GBM cells through inhibiting the thioredoxin system,https://civilica.com/doc/1186920

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