The Effect of GnRH and Naloxone on Serum Level Variations of FSH, LH and Testosterone Induced by Morphine Administration in Male wistar Rats.

Background & objective: With regard to increasing use of opioids and their potential role in infertility research centers around the world are in search of pharmacologic compounds which could neutralize effects of opioids and overcome infertility through administration of GnRH and its analogues which also do not have considerable side effects. Materials & Methods: In this study male wistar rats weighing ۲۰۰-۲۵۰ gr were used. At different intervals (۵-۱۰-۱۵ days) ۵mg/kg morphine was injected intraperitonneally into the male rats. Then they were divided in ۵ groups of ۸. The first group contained intact rats, while saline, morphine, naloxone, and fertagyl were injected into the second third, fourth and fifth groups respectively. Then the rats were anesthetized and their bloods were taken for further tests. Results: The results showed that morphine induces loss of testis weight and diameter, loss of weight in rats, and nutritional and behavior changes. Furthermore, a significant changes in the amounts of LH and testosterone hormones was observed in all groups (p<۰.۰۵) while no significant change in the amount of FSH was observed. Conclusion: Since the experimental groups into which naloxone and GnRH were injected showed no significant difference, we suggest that naloxone and GnRH be administrated as opioid antagonist to slove the problem of morphine-induced infertility.

Keywords:

Morphine , Naloxone , GnRH , LH , FSH , Testoterone