مرتضی پیری - Dept. of Biology, Islamic Azad University, Ardabil Branch, Ardabil, Iran
الهام ایازی - Dept. of Biology, Islamic Azad University, Tehran North Branch, Tehran, Iran
مریم بنانج - Dept. of Biology, Islamic Azad University, Tehran North Branch, Tehran, Iran
مریم السادات شاهین - Young Researchers Club, Islamic Azad University Shahr-e-rey Branch, Tehran, Iran

Background and Objective: Dopamine receptors and histamine influence anxiety-like behaviors. Furthermore, interaction between histamine and dopaminergic D۱ receptors has been demonstrated in the modulation of some behavior. In the present study, we investigated the interaction between histamine and dopaminergic D۱ receptors in the dorsal hippocampus in the anxiety behavior. Materials and Methods: In this experimental study, the elevated plus maze test was used to test anxiety-like behaviors. Mice were anesthetized with intra-peritoneal injection of ketamine hydrochloride, plus xylazine, and two stainless-steel cannuale were placed in the CA۱ region of the hippocampus. Two- and one-way analyses of variance (ANOVAs), followed by LSD test, were used for data analysis. All experiments were performed in accordance with the institutional guidelines for animal care and use. Results: Intra-CA۱ injection of histamine and D۱ dopamine agonist (SKF ۳۸۳۹۳) and antagonist (SCH۲۳۳۹۰) induced anxiety. Intra-CA۱ injection SKF ۳۸۳۹۳ or SCH۲۳۳۹۰ ۲ min after of effective dose of histamine (۱۰µg/mouse) inhibited anxiogenic effects of histamine. Conclusion: It seems that both histamine and dopaminergic D۱ receptors play a part in the modulation of anxiety in the dorsal hippocampus of mice, and that they carry a complex interaction between them.

Keywords: SKF ۳۸۳۹۳, SCH۲۳۳۹۰, Histamine, Anxiety, Elevated plus-maze, Mouse