Effect of Hydro-Alcoholic Extract of Dorema Aucheri on Serum Levels of Testosterone, FSH and Sperm Count in Nicotinamide-STZ- Induced Diabetic Rat Models

Publish Year: 1392
نوع سند: مقاله ژورنالی
زبان: English
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JR_ZUMS-21-87_003

تاریخ نمایه سازی: 11 اردیبهشت 1400

Abstract:

Background and Objective: Diabetes can lead to sexual dysfunction in patients. Recent studies indicate the influence of Dorema aucheri on sexual hormones. Therefore, we decided to elicit the effect of hydro-alcoholic extract of Dorema aucheri on serum levels of testosterone and FSH, testicular morphology and sperm count of Nicotinamide-STZ (streptozotocin) induced diabetes in rat models. Materials and Methods: A total of ۴۲ male adult wistar rats weighing ۱۵۰-۲۵۰gm were entered into the study. To produce diabetic model, the animals received one dose of nicotinamide۱۲۰ mg/kg and STZ۶۰ mg/kg intraperitoneally. The animals were divided into ۶ groups (۷rats in each group): control, diabetic, diabetic+glibenclamid (۰.۶mg/kg), as well as variant doses of hydro-alcoholic extract of Dorema aucheri (۱۰۰, ۲۰۰, ۴۰۰mg/kg). The extracts were gavaged once a day, ۵ days per week for ۳ weeks. ۲۴ hours after the last gavage under deep anesthesia with ether, blood samples were collected from the heart and then serum levels of testosterone and FSH were measured. Subsequently, testicular morphology and caudal epididymal sperm numbers were determined and counted. The data were statistically analyzed with SPSS by one-way ANOVA whereby P<۰.۰۵ was considered as significant. Results: Serum level of testosterone exhibited a significant increase in diabetic rats compared with all other groups except the control group (P=۰.۰۵). The ۲۰۰mg/kg extract concentration led to a significant increase in sperm counts in comparison with diabetic (P=۰.۰۰۳) and other groups (P<۰.۰۵). Conclusion: The results indicated that hydro-alcoholic extracts of Dorema aucheri improve sperm production process and increase sperm counts in rats. This positive effect may originate from the presence of flavonoids in Dorema aucheri plant.

Authors

اکرم آهنگرپور

Physiology and Diabete Research Center, School of Medicine, Jundishapur University of Medical Sciences, Ahvaz, Iran

علیاکبر عروجن

Dept. of Physiology, Student Research Committee, Ahvaz Jundishapur University of Medical Science, Ahvaz, Iran

حمید حیدری

Dept. of Physiology, Student Research Committee, Ahvaz Jundishapur University of Medical Science, Ahvaz, Iran