Effects of Salvia Officinalis L. Extract on Biochemical Blood Parameters in Male Rats

Background and Objective: salvia officinalis L. consists of, glycosides, flavonoids, and anthocyanins. Due to the increasing use of medicinal plants in treatment of diseases and their side effects on various organs, this study was conducted to evaluate the effect of salvia officinalis on biochemical blood parameters in male rats. Materials and Methods: In this experimental study, ۲۴ male Wistar rats were randomly assigned to four groups of ۶ animals each. The control group received normal saline and treatment groups received ۷۵, ۱۵۰ and ۳۰۰ mg/kg body weight of salvia extract for ۱۴ days. Body weight was measured in days ۰, ۷, and ۱۴. At the end of the experiment, serum levels of liver function enzymes such as AST and ALT, total proteins, albumin, creatinine, cholesterol and triglyceride were assessed. Results were analyzed using one- way ANOVA and Tukey test. Results: According to the results, plasma concentrations of protein, albumin and creatinine showed a significant increase (P<۰.۰۵) but, a significant decrease was observed in liver enzymes. No significant changes were observed in serum cholesterol, triglyceride levels and body weight in the control and salvia groups (P>۰.۰۵). Conclusion: The results indicated that Salvia Officinalis probably contains flavonoidic components with antioxidant effect leading to the increase in albumin and decrease in liver enzymes. References ۱- Katzung B, Masters S, Trevor A. Basic & clinical pharmacology. Newyork: Mc Graw-Hill Medical. ۲۰۱۱. ۲- Glisic SB, Ristic M, Skala D, Ivanovic J. Extraction of sage (salvia officinalis L.) by supercritical Co۲: kinetic data, chemical composition and selectivity of diterpenes. J Superitica Fluids. ۲۰۱۰ ۵۵(۱): ۶۲-۷۰. ۳- Oboh G, Henle T. Antioxidant and inhibitory effects of aqueous extracts of salvia officinalis leaves on pro-oxidant-induced lipid peroxidation in brain and liver in vitro. J Med Food. ۲۰۰۹ ۱۲(۱): ۷۷-۸۴. ۴- Wang M, Shao Y, Li J, et al. Antioxidative phenolic glycosides from sage (Salvia officinalis). J Nat Prod. ۱۹۹۹ ۶۲: ۴۵۴-۶. ۵- Baricevic D, Sosa S, Della Loggia R, et al. Topical anti in flammatory activity of Salvia officinalisl leaves. J. Ethnopharmacol. ۲۰۰۱ ۷۵: ۱۲۵-۳۲. ۶- Weiss RF, Fintelman Volker. Herbal Medicine Germany: Thieme Verlag. ۲۰۰۱. ۷- Orhan I, Kartal M, Naz Q, et al. Antioxidant and anticholinesterase evaluation of selected turkish salvia species. Food Chemistry. ۲۰۰۷: ۱۰۳: ۱۲۴۷-۵۴. ۸- Tepe B, Eminagaoglu O, Akpulat H, Aydin E. Antioxidant potentials and rosmarinic acid levels of the methanolic extracts of Salvia verticillata (L.) subsp. Verticillata and S. verticillata (L.) subsp. Amasiaca (Freyn & Bornm.) Bornm Food Chemistry. ۲۰۰۷ ۱۰۰: ۹۸۵-۹. ۹- Abd-Elmageed MAM, Hussein BA. Cytotoxicity and antimicrobial activity of Salvia officinalis L. flowers. Sudan JMS. ۲۰۰۸ ۳(۲): ۱۲۷-۳۳. ۱۰- Krinke GJ. History, strains and models. The laboratory rat Hand book of Experimental Animals. UK: Academic Press. ۲۰۰۰. ۱۱- Zhong QW, Zhang HX, Russeell JC, Hulver M, Cefalu WT. Chromium picolinate enhances skeletal muscle cellular- Resistant JCR: LA-cp Rats. J. Nutr. ۲۰۰۶ ۱۳۶: ۴۱۵-۲۰. ۱۲- Zhanq H, Chen L, Zhanq J, et al. Effect of Salvia miltiorrhizae on apoptosis and NF-kappaB p۶۵ expression in the liver of rats with severe acute pancreatitis or obstructive jaundice. J. Gastroenterol Hepatol. ۲۰۰۹ ۲۴(۵): ۸۴۱-۵۲. ۱۳- Zargari A. Pharmaceutical plants (persian). Tehran: Tehran University Press ۱۹۹۷. ۱۴- Sahin F, Gulluce M, Daferera D, et al. Biological activities of the essential oils and methanol extract of Origanum Vulgare in the Eastern Anatolia region of Turkey. Food Control. ۲۰۰۴ ۱۵ (۷): ۵۴۹-۵۷. ۱۵- Shishenian B, Farzin S. Medical hematology.Tehran: Daneshjo Press ۱۹۹۹. ۱۶- Thomas L. Clinical laboratory diagnostics. Frank Furt: TH-Books Verlagsgesell schaft. ۱۹۹۸. ۱۷- Mirzaee A, Hakimi MH, Sadeghi H. Total antioxidant activity and phenolic content of Dorema aucheri: Iran J Biocheme Mol Biol. ۲۰۰۵ ۱: ۱۱. ۱۸- Pizzale L, Bortolomeazzi R, Vichi S, Uberegger E, Conte LS. Antioxidant activity of sage (Salvia officinalis and S fruticosa) and oregano (Origanumonites and O indercedens) extracts related to their phenolic compound content. J Sci Food Agric. ۲۰۰۲ ۸۲: ۱۶۴۵-۵۱. ۱۹- Lu Y, Foo L.Y. Flavonoid and phenol glycosides from Salvia Officinalis. Photochemistry. ۲۰۰۰ ۵۵: ۲۶۳-۷. ۲۰- Esmaeili MA, Sombol A, Kanani M, Sadeghi H, Karimian Pour N. Evaluation of the effect of Salvia Sahandica on tissue damages induced by alcohol in oxidative stress conditions in the rat: effect on liver and kidney oxidative parameters. Pharmalogical Sciences. ۲۰۰۸ ۱۵: ۳۱۵-۲۲. ۲۱- Yurtseven S, Cetin M, Sengul T, Sogut B. Effect of sage extract (Salvia Officinalis) on growth performance, blood parameters, oxidative stress and DNA damage in partridges. South African Journal of Animal Science. ۲۰۰۸ ۳۸ (۲): ۱۴۵-۱۵۲. ۲۲- Cassarett Douls. Toxicology: The basic science of poison. ۶th ed. Newyork: McGraw-Hill ۲۰۰۱. ۲۳- Cristovao F, Patricia C, Paula B, Rosa M, Manuel F, Cristina p. Water and methanolic extracts of Salvia Officinalis protect HepG۲ cells from t-BHP induced oxidative damage. Chemico-Biological Interactions. ۲۰۰۷ ۱۶۷: ۱۰۷-۱۵. ۲۴- Khakpour Sh, Khosravi M, Tajaddod G, et al. Evaluation of the effect of Salvia officinalis extract on liver in the rat. Tehran: Tehran University Press ۲۰۱۳. ۲۵- Soochan D, Keough V, Wanless I, Molinari M. Intra and extra-hepatic cystadenoma of the biliary duct. Review of literature and radiological and pathological characteristics of a very rare case. BMJ Case Rep. ۲۰۱۲ ۲۵: ۱۱۰-۶. ۲۶- Hall JE. Guyton and Hall textbook of medical physiology. ۱۲th ed. New York: Saunders ۲۰۱۰.

Keywords:

Keywords: Liver enzymes , Blood parameters , Salvia extract , Male rat