Elaidic acid Effects on Osteonectin Gene Expression in Vascular Smooth Muscle Cells

Background and Objective: Atheroma formation and progression of atherosclerosis are dependent on the expression of bone matrix proteins and regulatory factors such as osteonectin in the vessel walls. Studies have shown that consumption of Trans fatty acids increase risk of cardiovascular diseases. In this study, the effect of elaidic acid on osteonectin gene expression as one of the vascular calcification factors was investigated. Materials and Methods: Vascular smooth muscle cells were treated with concentrations of ۵, ۱۰ and ۲۰ µM of elaidic acid for ۴۸h and compared with control group. Total RNA was extracted and cDNA was synthesized and then the quantity of osteonectin gene expression was measured by real time PCR. 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Keywords:

Keywords: Vascular smooth muscle cells , Elaidic acid , Osteonectin , Atherosclerosis