Effects of Isoproterenol (beta-adrenergic agonist) on in vitro differentiation of human mesenchymal stem cells into osteoblasts

Background and Objective: The importance of β-adrenergic signals in bone formation and resorption has been well investigated. However, little is known about the role of β -adrenergic signals in osteoblastic differentiation of mesenchymal stem cells (MSCs), which is critically important in bone physiology and pharmacology. In this study, RUNX۲ and Osteocalcin gene expression were quantified in MSCs differentiated by osteoblastic differentiation medium (ODM) and Isoproterenol (ISO). Materials and Methods: In this experimental study, human mesenchymal stem cells were treated by osteoblastic differentiation medium and ISO. RNA extraction was carried out from both osteoblastic differentiation medium at ۴ and ۲۱ days of differentiation and from undifferentiated MSCs. RUNX۲ and osteocalcin gene expression were quantified by quantitative Real Time-PCR. Results: Isoproterenol decreased the expression of RUNX۲ and osteocalcin genes at ۴ and ۲۱ days of osteoblastic differentiation. 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Keywords:

Keywords: Mesenchymal stem cells , Osteoblastic differentiation Medium , Beta adrenergic , Isoproterenol , RUNX۲ , Osteocalcin