The effect of mimicking the niche of placenta with PLLA Scaffold on expansion of hematopoietic stem cells derived from human placenta

Background and Objective: Nowadays, although umbilical cord blood is a commonly used source of hematopoietic stem cell, its low frequency of these cells is the main factor limiting its clinical application. The transplantation of hematopoietic stem cells derived from placenta tissue along with umbilical cord blood cells of the same sample may be an appropriate approach to solve this problem. In this study, we tried to mimic the niche of placenta by nano fiber scaffold in order to expand the hematopoietic stem cells derived from placenta tissue. Materials and Methods: Different stromal cells along with hematopoietic stem cells derived from placenta tissue were seeded on nano fiber scaffold produced from PLLA coated with collagen. Then, the rate of proliferation in these niches was studied. Results: The expansion in the mimicked niche associated with ۳.۶ fold increase in the number of cells but the capacity of forming colonies decreased significantly (P<۰.۰۰۰۱). 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Keywords: Placenta , Hematopoietic stem cells , Stem cells niche , Nano fiber scaffold