داوود مهدیان - Dept. of Pharmacology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
آذر حسینی - Pharmacological Research Center of Medicinal Plants, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
حسن رخشنده - Pharmacological Research Center of Medicinal Plants, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

Background and Objective: Doxorubicin is one of the most effective chemotherapeutic agents against solid tumors. Doxorubicin causes cardio-toxicity via production of free radicals and depletion of anti-oxidants. However, the use of anti-oxidants can decrease doxorubicin-induced cardio-toxicity. Apium graveolens (celery) contains anti-oxidant compounds. Hence, this study was an attempt to figure out if it can protect heart cells against oxidative stress of doxorubicin. Materials and Methods: The cells were incubated with different concentrations of Apium graveolens (celery) extract for ۴ hours which continued in the presence of ۵µM doxorubicin for ۲۴ hours. Cell viability and the apoptotic induction were determined using ۳-(۴,۵-dimethylthiazol-۲-yl)-۲,۵ diphenyl tetrazolium (MTT) and propidium iodide (PI) staining assays, respectively. Results: Celery extract increased toxicity of doxorubicin at a concentration of ۱۲۵-۵۰۰ µg/ml. Also apoptosis was induced in the presence of doxorubicin at a concentration of ۲۵۰ and ۵۰۰µg/ml. Conclusion: Results showed that celery has no toxicity effect on heart cells alone. However, this extract could not protect cells against doxorubicin- induced cardiotoxicity. A number of mechanisms may instigate this effect such as augmentation of free radicals, increased drug delivery into the cells and expression of apoptotic proteins. Further research is needed to decide upon accuracy mechanism.

Keywords: Doxorubicin, H۹c۲, Apoptosis, Apium graveolens